ABL1 Antibody (LS-C20250)
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Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Rabbit anti-Mouse Polyclonal (aa929-1020) Antibody - LS-C20250 - LSBio |
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Antibody Details
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| Antibody Catalog ID: |
LS-C20250 |
| Antibody Target: |
Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) |
| Synonyms: |
ABL1, Abl, Bcr-Abl, c-abl, jtk7, p150, proto-oncogene tyrosine-protein kinase abl, v-abl, v-abl abelson murine leukemia viral oncogene homolog 1 |
| Family: |
Protein Kinase |
| Subfamily: |
Abl |
| Host: |
ABL1 antibody was produced in Rabbit |
| Antigen Species: |
ABL1 antibody was raised against Mouse |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Antigen Type: |
Recombinant protein |
| Immunogen: |
ABL1 antibody was raised against recombinant protein containing Glutathione S-transferase fused to the SH3 domain of Abl-amino acids 929-1020. |
| Antibody Specificity: |
Recognizes human p210 bcr-abl, p140 c-Abl, as well as viral p160 gag-abl.
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| Antigen Modification: |
aa929-1020
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| Predicted Species Reactivity: |
Human
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| Purification: |
Protein G Column |
| Presentation: |
0.1 M Tris-glycine buffer, pH 7.4. |
| Recommended Storage: |
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: |
Western. Western Blot Analysis: This lot was tested by using the antibody (1 ug/ml) to detect Abl-Bcr (~210kD) and c-Abl (~140kD) in human K562 leukemia cells and NFC murine erythroleukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raji and Hut lymphoid cell lines, or murine Wehi myeloid cell line. Immunoprecipitation: 15 ug immunoprecipitates Abl-Bcr (~210kD) and c-Abl (~140kD) from a lysate of human K562 leukemia cells. |
| Antibody Uses: |
Western blot (WB), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: |
250 µg |
| Antibody Price (USD): |
$485 |
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Related Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibodies (37)
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| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-B1166 | Rabbit IgG | | Tyr412 | IHC-P | Human, Mouse | 50 µg | $350 |  |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-B2776 | Mouse IgG1 | | | WB, IP, IF, IHC-P | Human, Mouse | 50 µg | $350 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C33822 | Mouse IgG1 | | | IHC, WB, IP, ELISA, IF | Mouse, Human | 50 µg | $295 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C33821 | Rabbit | | | IHC, WB, IP, ELISA | Human, Mouse | 50 µg | $295 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C88554 | Mouse IgG1 | | SH2 Domain | WB, IP, IF, IHC-P | Human, Mouse | 20 µg | $250 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C88555 | Mouse IgG1 | Biotin | | WB, IP, IF, IHC-P | Human, Mouse | 20 µg | $280 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C97399 | Rabbit | | pTyr134 | WB, ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C97400 | Rabbit | | pTyr245 | WB, ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C97401 | Rabbit | | pTyr251 | WB, ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C99160 | Rabbit | | C-Terminus | WB, ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C99162 | Rabbit | | Internal | WB, ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C97398 | Rabbit | | pSer588 | IHC, ELISA | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C97402 | Rabbit | | pTyr272 | ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C97403 | Rabbit | | pTyr276 | ELISA, IHC-P | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C51272 | Rabbit IgG | | pTyr412 | IHC | Human, Mouse | 40 µg | $245 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C20984 | Rabbit IgG | | C-Terminus | ICC, IHC, IP | Human | 100 µl | $445 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C77356 | Rabbit IgG | | | WB, IP, IHC-P | Human, Mouse | 500 µl | $470 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C77351 | Rabbit IgG | | | IHC-P | Human, Mouse | 7000 µl | $480 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C49588 | Rabbit IgG | | | WB, IP | Human, Rat | 100 µl | $350 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C109117 | Mouse IgG1 | | | WB, ELISA | Human | 100 µl | $320 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C53734 | Rabbit | | pTyr245 | WB | Human, Mouse | 10 blot | $488 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C53735 | Rabbit | | pTyr412 | WB | Human, Mouse | 10 blot | $488 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C45601 | Mouse IgG1 | | | WB, IP, IF | Human, Mouse | 100 µg | $365 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C99159 | Rabbit | | N-Terminus | WB, ELISA | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C99161 | Rabbit | | N-Terminus | WB, ELISA | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C96814 | Rabbit | | pTyr204 | ELISA | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C96835 | Rabbit | | pTyr283 | ELISA | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C96836 | Rabbit | | pThr754 | ELISA | Human | 100 µg | $267 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C12193 | Rabbit IgG | | N-Terminus | ICC, WB, Flo, ELISA | Human, Mouse, Rat | 100 µl | $405 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C12199 | Rabbit IgG | | C-Terminus | WB, IP, ELISA, IF | Human, Mouse, Rat | 100 µl | $405 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C12200 | Rabbit IgG | | pTyr245 | WB, ELISA | Human, Mouse | 100 µl | $440 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C20249 | Rabbit IgG | | | WB, IP | Mouse, Human | 250 µg | $485 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C34784 | Rabbit IgG | | | WB, Func | | 200 µg | $485 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C12201 | Rabbit | | pTyr245 | WB | Human | 100 µl | $500 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C12202 | Rabbit IgG | | pThr735 | WB | Human | 100 µl | $430 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C77349 | Mouse IgG2a | | | WB | Human, Mouse, Rat | 100 µl | $450 | |
| Abelson Murine Leukemia Viral Oncogene Homolog 1 (ABL1) Antibody | LS-C77354 | Rabbit IgG | | pTyr412 | WB | Human | 100 µl | $480 | |
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Antibody Summary:
The v-abl oncogene, which was originally described in the murine Abelson retrovirus, encodes a non-receptor tyrosine kinase that can mediate cell transformation (1-3). Mammalian c-Abl is associated with normal cell growth and differentiation, and is distributed in the nucleus, and the cytoplasm in association with F-actin. In the nucleus, c-Abl has been shown to bind chromatin in a cell cycle regulated manner. Deletion of the F-actin binding and the chromatin binding regions of c-Abl has been shown to block cell growth despite the production of an active tyrosine kinase. In chronic myelogenous leukemia (CML), the c-abl protooncogene translocates from chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22 forming the Philadelphia chromosome (4). This translocation causes the expression of a novel 210kD Abl-Bcr fusion gene product which is only found in malignant cells and is believed to play a crucial role in the pathogenesis of CML. Western Immunoblot Analysis This lot was tested by using the antibody (1ug/ml) to detect Abl-Bcr (~ 210kDa) and c-Abl (~ 140kDa) in human K562 leukemia cells and NFC murine erythroleukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj and Hut lymphoid cell lines, or murine Wehi myeloid cell line. Immunoprecipitation 15ug immunoprecipitates Abl-Bcr (~210kDa) and c-Abl (~ 140kDa) from a lysate of human K562 leukemia cells. Recognizes human p210 bcr-abl , p140 c-Abl , as well as viral p160 gag-abl.
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| Requested From: |
United States |
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