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RELA Antibody

V-rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) Rabbit anti-Human Polyclonal (N-Terminus) Antibody - LS-C18905 - LifeSpan BioSciences

Antibody Technical Query IHC Request For Information Buy This Antibody
Antibody Catalog ID: LS-C18905
Antibody Target: V-rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)
Synonyms: RELA, nfkb3, nuclear factor kappa-b, subunit 3, transcription factor p65, v-rel avian reticuloendotheliosis viral oncogene homolog a
Host: RELA antibody was produced in Rabbit
Antigen Species: RELA antibody was raised against Human
Clonality: Polyclonal
Antibody Specificity: The NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Antigen Modification: N-Terminus
Purification: Immunoaffinity Chromatography
Presentation: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Recommended Storage: Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
Antibody Usage Summary: Suitable for immunohistochemistry, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB p65 NLS at a dilution of 1:2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NFxB NLS is suitable for the gel supershift assay and was found to be reactive against all p65 (Rel A) containing human NFxB complexes using 0.5 to 1.0 ul per assay.
Antibody Uses: Immunohistochemistry (IHC) (1:200 ), Western blot (WB) (1:2000 ), Immunofluorescence (IF) (1:200 ), ELISA (Optimal dilution to be determined by the researcher)
Size: 100 µg
Antibody Price (USD): $345

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Antibody Summary:
NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. IkB-a binding masks the NFkB nuclear localisation signal (NLS). A broad range of external stimuli lead to activation of NFkB and set off signalling cascades that ultimately converge on the IkB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IkB-a and this phophorylation event targets IkB-a for degradation. As a consequence, NFkB NLS is uncovered and nuclear translocation occurs.
Requested From: United States

For Research Use Only. Not intended for human, diagnostic or therapeutic purposes.
The data on this page have been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).
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