| Antibody Catalog ID: |
LS-B2541 |
| Antibody Validation: |
This antibody replaces catalog number LS-C17345. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: |
AKT1 Substrate 1 (proline-rich) (AKT1S1) |
| Synonyms: |
AKT1S1 |
| Host: |
AKT1S1 antibody was produced in Mouse |
| Antigen Species: |
AKT1S1 antibody was raised against Human |
| Clonality: |
Monoclonal |
| Isotype: |
IgG1,k |
| Antigen Type: |
Recombinant protein |
| Immunogen: |
AKT1S1 antibody was raised against recombinant full length human PRAS40 expressed in E. coli. |
| Antibody Specificity: |
Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
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| Purification: |
Protein A/G Column |
| Presentation: |
PBS, pH 7.2, 0.1% sodium azide, 1% BSA. |
| Recommended Storage: |
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: |
Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells. |
| Antibody Uses: |
Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml ), Western blot (WB) (0.1 - 1 µg/ml ), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: |
50 µg |
| Antibody Price (USD): |
$350 |
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Immunohistochemistry Image:
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| Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) |
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Related AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibodies (8)
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| Target | Catalog | Type | Form | Epitope | Assay | Reactivity | Size | Price | Buy |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C53893 | Rabbit | | pThr246 | | Human | 50 µl | $456 |  |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C81149 | Rabbit IgG | | | | Human | 50 µg | $380 | |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C75035 | Rabbit IgG | | aa195-245 | | Human | 20 µg | $535 | |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C38305 | Rabbit | | pThr246 | | Human | 100 µl | $505 | |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C75033 | Rabbit IgG | | | | Human | 100 µl | $405 | |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C75034 | Rabbit IgG | | aa225-256 | | Human | 100 µg | $535 | |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C75036 | Mouse IgG1 | | | | Human | 100 µg | $505 | |
| AKT1 Substrate 1 (proline-rich) (AKT1S1) Antibody | LS-C92259 | Rabbit IgG | AF488 | pThr246 | | Human | 100 µl | $535 | |
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Antibody Summary:
Proline-Rich AKT Substrate (PRAS40) is a 40kD substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. AKT and PRAS40 are components of the phosphoinositide 3-kinase (PI3K) pathway. This pathway plays a role in glucose uptake, cell growth and apoptosis inhibition. The precise function of PRAS40 is not yet known; however, it has been hypothesized that PRAS40 interacts with SH3 and WW domain containing proteins and may change the function of these proteins. Suitable for use in Western Blot, Immunohistochemistry (FFPE), and immunoprecipitation. Other applications not tested. Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
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| Requested From: |
United States |