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See All 16 ABCB5 Antibodies

Ordering
Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $385

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866-206-6909
antibodies@lsbio.com
 

ABCB5 Antibody LS-B3454 Images

Immunohistochemistry
Anti-ABCB5 antibody IHC staining of human breast.
Western Blot
Western blot analysis of K562 cell line lysates (35 ug/lane).
Flow Cytometry
Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# LS-B3454 on SkMel-30 cell line.
Flow Cytometry
Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# LS-B3454 on WM852 cell line.

Anti-ABCB5 Antibody LS-B3454 IHC-plus™

Rabbit anti-Human Polyclonal, N-Terminus, Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)

Details

Applications
Human ABCB5
Rabbit
Human
Polyclonal
Ammonium Sulfate Precipitation
Unconjugated (Unconjugated)
IHC-P (10 µg/ml)
Western blot (1:50 - 1:100)
Flo
Optimal dilution to be determined by the researcher.

Specificity and Use

ABCB5 antibody was raised against kLH conjugated synthetic peptide selected from the N-terminal region of human ABCB5.
N-Terminus
Immunohistochemistry: LS-B3454 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3454 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells. Freeman JB, Gray ES, Millward M, Pearce R, Ziman M. Journal of translational medicine. 2012 10:192. [PubMed:22978632] [PMC:PMC3480925]

About ABCB5

Q2M3G0 BC044248

ABCB5 Antibody, ABCB5alpha Antibody, ABCB5 P-gp Antibody, ABCB5beta Antibody, ATP-binding cassette protein Antibody, EST422562 Antibody, P-glycoprotein ABCB5 Antibody

(More About ABCB5)

ABCB5 Antibodies (16)

Target Host Clonality Format Epitope Reactivity Applications
ABCB5 Rabbit Polyclonal Human Flo, IHC-P, WB
ABCB5 Antibody LS-B3454 Rabbit Polyclonal thumb(4)
50 µg - 385.00
Applications: IHC-P, WB, Flo Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
ABCB5 Rabbit Polyclonal Human IHC-P, WB
ABCB5 Antibody LS-B6371 Rabbit Polyclonal thumb(1)
50 µl - 385.00
Applications: IHC-P, WB Epitope:  
Reactivity: Hu Format: Unconjugated  
ABCB5 Mouse Monoclonal Human ELISA, Flo, ICC, IHC, WB
ABCB5 Antibody [5H3C6] LS-C169144 Mouse Monoclonal thumb(7)
100 µg - 350.00
Applications: IHC, ICC, WB... Epitope:  
Reactivity: Hu Format: Unconjugated  
ABCB5 Rabbit Polyclonal Human ELISA, IHC, WB
ABCB5 Antibody LS-C154236 Rabbit Polyclonal thumb(2)
100 µg - 380.00
Applications: IHC, WB, ELISA Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
ABCB5 Rabbit Polyclonal Human, Mouse ICC, IHC-P
ABCB5 Antibody LS-C180152 Rabbit Polyclonal thumb(2)
100 µl - 335.00
Applications: IHC-P, ICC Epitope:  
Reactivity: Hu, Mu Format: Unconjugated  
ABCB5 Rabbit Polyclonal Human, Mouse ICC, IHC-P
ABCB5 Antibody LS-C180804 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC Epitope:  
Reactivity: Hu, Mu Format: Biotin  
ABCB5 Rabbit Polyclonal Human, Mouse ICC, IHC-P
ABCB5 Antibody LS-C180805 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC Epitope:  
Reactivity: Hu, Mu Format: DY650  
ABCB5 Rabbit Polyclonal Human, Mouse ICC, IHC-P
ABCB5 Antibody LS-C180806 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC Epitope:  
Reactivity: Hu, Mu Format: DY488  
ABCB5 Rabbit Polyclonal Human, Mouse ICC, IHC-P
ABCB5 Antibody LS-C180807 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC Epitope:  
Reactivity: Hu, Mu Format: DY550  
ABCB5 Goat Polyclonal Human ELISA, WB
ABCB5 Antibody LS-C61699 Goat Polyclonal thumb(1)
100 µg - 295.00
Applications: WB, ELISA Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
ABCB5 Rabbit Polyclonal Human ICC
ABCB5 Antibody LS-C180154 Rabbit Polyclonal thumb(1)
100 µl - 335.00
Applications: ICC Epitope:  
Reactivity: Hu Format: Unconjugated  
ABCB5 Goat Polyclonal Bovine, Dog, Human, Mouse, Rat ELISA
ABCB5 Antibody LS-C154903 Goat Polyclonal
100 µg - 295.00
Applications: ELISA Epitope: aa364-378  
Reactivity: Bo, Do, Hu ... Format: Unconjugated  
ABCB5 Rabbit Polyclonal Human ICC
ABCB5 Antibody LS-C180813 Rabbit Polyclonal
100 µl - 365.00
Applications: ICC Epitope:  
Reactivity: Hu Format: Biotin  
ABCB5 Rabbit Polyclonal Human ICC
ABCB5 Antibody LS-C180814 Rabbit Polyclonal
100 µl - 365.00
Applications: ICC Epitope:  
Reactivity: Hu Format: DY650  
ABCB5 Rabbit Polyclonal Human ICC
ABCB5 Antibody LS-C180815 Rabbit Polyclonal
100 µl - 365.00
Applications: ICC Epitope:  
Reactivity: Hu Format: DY488  
ABCB5 Rabbit Polyclonal Human ICC
ABCB5 Antibody LS-C180816 Rabbit Polyclonal
100 µl - 365.00
Applications: ICC Epitope:  
Reactivity: Hu Format: DY550  

Immunohistochemistry

Immunohistochemistry
Anti-ABCB5 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3454 concentration 10 ug/ml.

Western Blot

Western Blot
Western blot analysis of K562 cell line lysates (35 ug/lane).

Flow Cytometry

Flow Cytometry
Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# LS-B3454 on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver

Flow Cytometry

Flow Cytometry
Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# LS-B3454 on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
Requested From: United States
Date Requested: 5/25/2013
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