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This antibody may not be available in some countries due to import restrictions. Please inquire before purchase.
See All 18 Osteonectin / SPARC Antibodies

Ordering
Wt. Vol. Conc. Price
100 µg - - $395

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206-374-1102
866-206-6909
antibodies@lsbio.com
 

Osteonectin / SPARC Antibody LS-C150076 Images

Immunohistochemistry
SPARC/Osteonectin in Human Ovary.
Western Blot
Detection of Human SPARC by Western Blot.

Anti-Osteonectin / SPARC Antibody LS-C150076

Goat anti-Human Polyclonal, Unconjugated

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Details

Applications
Human Osteonectin / SPARC
Goat
Human
IgG Polyclonal
Immunoaffinity Chromatography
Unconjugated (Unconjugated)
IHC (5 - 15 µg/ml)
Western blot (1 µg/ml)
Flo
Optimal dilution to be determined by the researcher.

Specificity and Use

Osteonectin / SPARC antibody was raised against mouse myeloma cell line NS0-derived recombinant human SPARC/Osteonectin Ala18-Ile303 Accession # P09486.
Detects human SPARC/Osteonectin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with.
Western Blot, 1 µg/ml. Immunohistochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells, MG-63 human osteosarcoma cell line fixed with paraformaldehyde and permeabilized with. saponin.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About Osteonectin / SPARC

P09486 NM_003118 NP_003109.1

SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody

SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and.
(More About Osteonectin / SPARC)

Osteonectin / SPARC Antibodies (18)

Target Host Clonality Format Epitope Reactivity Applications
Osteonectin / SPAR... Mouse Monoclonal Bovine, Human, Rat ELISA, IHC, RIA, WB
Osteonectin / SPARC Antibody LS-C25800 Mouse Monoclonal
100 µg - 325.00
Applications: IHC, WB, ELISA... Epitope:  
Reactivity: Bo, Hu, Rt Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human Flo, IHC, WB
Osteonectin / SPARC Antibody LS-C161424 Rabbit Polyclonal thumb(4)
100 µg - 290.00
Applications: IHC, WB, Flo Epitope: aa230-260  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Goat Polyclonal Human Flo, IHC, WB
Osteonectin / SPARC Antibody LS-C150076 Goat Polyclonal thumb(2)
100 µg - 395.00
Applications: IHC, WB, Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Goat Polyclonal Mouse Flo, IHC, WB
Osteonectin / SPARC Antibody LS-C150257 Goat Polyclonal thumb(1)
100 µg - 395.00
Applications: IHC, WB, Flo Epitope:  
Reactivity: Mu Format: Unconjugated  
Osteonectin / SPAR... Mouse Monoclonal Human IHC-Fr, IHC-P, WB
Osteonectin / SPARC Antibody LS-C83590 Mouse Monoclonal
100 µg - 440.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Rat Monoclonal Mouse ELISA, Flo, IHC-Fr, IHC-P
Osteonectin / SPARC Antibody LS-C124320 Rat Monoclonal
500 µg - 530.00
Applications: IHC-Fr, IHC-P, Flo... Epitope:  
Reactivity: Mu Format: Unconjugated  
Osteonectin / SPAR... Mouse Monoclonal Human Flo, IHC-P, WB
Osteonectin / SPARC Antibody [LM2] LS-C140650 Mouse Monoclonal
100 µg - 330.00
Applications: IHC-P, WB, Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Mouse Monoclonal Bovine, Human ELISA, Flo, IHC, WB
Osteonectin / SPARC Antibody LS-C25804 Mouse Monoclonal
100 µg - 480.00
Applications: IHC, WB, Flo... Epitope:  
Reactivity: Bo, Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human ELISA, IHC-P
Osteonectin / SPARC Antibody LS-C25802 Rabbit Polyclonal
100 µl - 465.00
Applications: IHC-P, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human ELISA, IHC-P
Osteonectin / SPARC Antibody LS-C122256 Rabbit Polyclonal
20 µl - 350.00
Applications: IHC-P, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human WB
Osteonectin / SPARC Antibody LS-C26929 Rabbit Polyclonal thumb(1)
100 µg - 360.00
Applications: WB Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human WB
Osteonectin / SPARC Antibody LS-C145377 Rabbit Polyclonal thumb(1)
50 µg - 380.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Mouse Monoclonal Human WB
Osteonectin / SPARC Antibody LS-C156115 Mouse Monoclonal thumb(1)
100 µl - 290.00
Applications: WB Epitope: aa224-251  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human WB
Osteonectin / SPARC Antibody LS-C26933 Rabbit Polyclonal
70 µg - 460.00
Applications: WB Epitope:  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Goat Polyclonal Mouse ELISA, Flo, WB
Osteonectin / SPARC Antibody LS-C131158 Goat Polyclonal
100 µg - 560.00
Applications: WB, Flo, ELISA Epitope:  
Reactivity: Mu Format: Unconjugated  
Osteonectin / SPAR... Mouse Monoclonal Bovine, Human ELISA, WB
Osteonectin / SPARC Antibody LS-C25808 Mouse Monoclonal
100 µg - 390.00
Applications: WB, ELISA Epitope:  
Reactivity: Bo, Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human ELISA
Osteonectin / SPARC Antibody LS-C124319 Rabbit Polyclonal
20 µl - 350.00
Applications: ELISA Epitope: aa272-286  
Reactivity: Hu Format: Unconjugated  
Osteonectin / SPAR... Rabbit Polyclonal Human ELISA
Osteonectin / SPARC Antibody LS-C25805 Rabbit Polyclonal
20 µl - 360.00
Applications: ELISA Epitope: aa272-286  
Reactivity: Hu Format: Unconjugated  

Immunohistochemistry

Immunohistochemistry
SPARC/Osteonectin in Human Ovary. SPARC/Osteonectin was detected in immersion fixed paraffin-embedded sections of human ovary using Goat Anti-Human SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody at 3 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western Blot

Western Blot
Detection of Human SPARC by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human SPARC/Osteonectin Antigen Affinity purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for SPARC at approximately 43 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: United States
Date Requested: 5/20/2013
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