Optimal dilution to be determined by the researcher.
Specificity and Use
ASM / SMPD1 antibody was raised against synthetic peptide from internal region of human SMPD1 (NP_000534.3). Percent identity by BLAST analysis: Human, Sheep, Dog, Rabbit, Horse, Pig (100%); Mouse, Rat (85%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.