LSBio The Immunohistochemistry Antibody Company
  • Home
  • Products
    • All Antibodies
    • IHC-plus Antibodies
  • Services
    • Immunohistochemistry Studies
    • Tissue Cross-Reactivity Screening
  • Distributors
  • Resources
    • About our Tissue Bank
    • Customer Support
    • How to Buy
    • IHC-plus Protocol
    • LSBio Media
    • Publications
    • Antibody Reports
  • Company
    • About LifeSpan
    • Press Releases
    • Holiday Schedule
    • Contact Us
  • View Cart

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $385

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

SMAD3 Antibody LS-B2064 Images

Immunohistochemistry
Anti-SMAD3 antibody IHC staining of human adrenal.

Anti-SMAD3 Antibody LS-B2064 IHC-plus™

Rabbit anti-Human Polyclonal, aa100-150, Unconjugated

100% Guaranteed 100% Guaranteed

Details

Applications

Human SMAD3
Rabbit
Human, Bovine, Dog, Macaque, Monkey, Mouse, Pig, Rat
Polyclonal
Protein G Column
Unconjugated (Unconjugated)
IHC-P (10 µg/ml)
Western blot (5 - 2 µg/ml)
Optimal dilution to be determined by the researcher.

Specificity and Use

aa100-150
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Immunohistochemistry: LS-B2064 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2064 was determined to be 10 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

SMAD3, a transcriptional regulator, is activated by TGF-beta and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC.


SMAD3 Antibodies (49)

Target Host Clonality Format Epitope Reactivity Applications
SMAD3 Rabbit Polyclonal Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish ELISA, IF, IHC-P, WB
SMAD3 Antibody LS-B64 Rabbit Polyclonal thumb(1)
50 µg - 385.00
Applications: IHC-P, IF, WB... Epitope: pSer423/425  
Reactivity: Bo, Ck, Fr ... Format: Unconjugated  
SMAD3 Mouse Monoclonal Human, Rat ELISA, IHC-P, WB
SMAD3 Antibody [7F3] LS-B5459 Mouse Monoclonal thumb(5)
50 µg - 450.00
Applications: IHC-P, WB, ELISA Epitope:  
Reactivity: Hu, Rt Format: Unconjugated  
SMAD3 Mouse Monoclonal Human ELISA, IHC-P, WB
SMAD3 Antibody [2C12] LS-B5455 Mouse Monoclonal thumb(4)
50 µg - 450.00
Applications: IHC-P, WB, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
SMAD3 Mouse Monoclonal Human, Mouse, Rat ELISA, IHC-P, IP, WB
SMAD3 Antibody [AF9F7] LS-B2555 Mouse Monoclonal thumb(2)
50 µl - 385.00
Applications: IHC-P, WB, IP... Epitope:  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Bovine, Dog, Human, Macaque, Monkey, Mouse, Pig, Rat IHC-P, WB
SMAD3 Antibody LS-B2064 Rabbit Polyclonal thumb(1)
50 µg - 385.00
Applications: IHC-P, WB Epitope: aa100-150  
Reactivity: Bo, Do, Hu ... Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human Flo, IF, IHC, WB
SMAD3 Antibody LS-C161298 Rabbit Polyclonal thumb(4)
100 µg - 290.00
Applications: IHC, IF, WB... Epitope: S208  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human Flo, IF, IHC, WB
SMAD3 Antibody LS-C161297 Rabbit Polyclonal thumb(5)
100 µg - 290.00
Applications: IHC, IF, WB... Epitope:  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human IF, IHC, WB
SMAD3 Antibody LS-C161296 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC, IF, WB Epitope: aa126-156  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Monoclonal Human, Mouse, Rat ICC, IHC-P, IP, WB
SMAD3 Antibody [EP568Y] LS-C49700 Rabbit Monoclonal thumb(2)
100 µl - 385.00
Applications: IHC-P, ICC, WB... Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish ELISA, IHC, WB
SMAD3 Antibody LS-C18974 Rabbit Polyclonal thumb(2)
100 µg - 345.00
Applications: IHC, WB, ELISA Epitope: aa417-425  
Reactivity: Bo, Ck, Fr ... Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC-P, WB
SMAD3 Antibody LS-C117540 Rabbit Polyclonal thumb(2)
100 µg - 295.00
Applications: IHC-P, WB, ELISA Epitope: C-Terminus  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC-P, WB
SMAD3 Antibody LS-C117931 Rabbit Polyclonal thumb(2)
100 µg - 295.00
Applications: IHC-P, WB, ELISA Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human ELISA, IHC, WB
SMAD3 Antibody LS-C178987 Rabbit Polyclonal thumb(2)
100 µl - 195.00
Applications: IHC, WB, ELISA Epitope: aa1-284  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC, WB
SMAD3 Antibody LS-C177436 Rabbit Polyclonal thumb(2)
100 µl - 345.00
Applications: IHC, WB Epitope: Met200  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC, WB
SMAD3 Antibody LS-C176302 Rabbit Polyclonal thumb(2)
100 µl - 345.00
Applications: IHC, WB Epitope: Pro417  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC, WB
SMAD3 Antibody LS-C176639 Rabbit Polyclonal thumb(2)
100 µl - 345.00
Applications: IHC, WB Epitope: Pro209  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human IHC-P, WB
SMAD3 Antibody LS-C96971 Rabbit Polyclonal thumb(2)
100 µg - 290.00
Applications: IHC-P, WB Epitope: pSer213  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Chicken, Dog, Human, Mouse, Pig, Rat, Xenopus, Zebrafish IHC, WB
SMAD3 Antibody LS-C31014 Rabbit Polyclonal thumb(2)
100 µg - 335.00
Applications: IHC, WB Epitope: N-Terminus  
Reactivity: Ck, Do, Fr ... Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC, WB
SMAD3 Antibody LS-C33662 Rabbit Polyclonal thumb(1)
50 µg - 295.00
Applications: IHC, WB Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Mouse Monoclonal Human, Mouse ELISA, ICC, WB
SMAD3 Antibody [5G11] LS-C169223 Mouse Monoclonal thumb(2)
100 µl - 330.00
Applications: ICC, WB, ELISA Epitope:  
Reactivity: Hu, Mu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC, WB
SMAD3 Antibody LS-C177435 Rabbit Polyclonal thumb(1)
100 µl - 345.00
Applications: IHC, WB Epitope: Asn175  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human ELISA, IHC, WB
SMAD3 Antibody LS-C10221 Rabbit Polyclonal
100 µg - 455.00
Applications: IHC, WB, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Bovine, Chicken, Fish , Human, Mouse, Pig, Rat, Zebrafish ELISA, IHC-P, WB
SMAD3 Antibody LS-C122429 Rabbit Polyclonal
50 µg - 590.00
Applications: IHC-P, WB, ELISA Epitope: aa417-425  
Reactivity: Bo, Ck, Fi ... Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC-P, WB
SMAD3 Antibody LS-C89107 Rabbit Polyclonal
500 µl - 510.00
Applications: IHC-P, WB Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC
SMAD3 Antibody LS-C124554 Rabbit Polyclonal
50 µg - 380.00
Applications: IHC Epitope: pSer425  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC
SMAD3 Antibody LS-C124555 Rabbit Polyclonal
50 µg - 345.00
Applications: IHC Epitope:  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC
SMAD3 Antibody LS-C124556 Rabbit Polyclonal
50 µg - 345.00
Applications: IHC Epitope:  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC
SMAD3 Antibody LS-C89103 Rabbit Polyclonal
40 µg - 305.00
Applications: IHC Epitope: Ser425  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat IHC
SMAD3 Antibody LS-C89104 Rabbit Polyclonal
40 µg - 305.00
Applications: IHC Epitope: Ser208  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human IHC
SMAD3 Antibody LS-C89105 Rabbit Polyclonal
40 µg - 320.00
Applications: IHC Epitope: pSer425  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse WB
SMAD3 Antibody LS-C99968 Rabbit Polyclonal thumb(2)
100 µg - 290.00
Applications: WB Epitope: Internal  
Reactivity: Hu, Mu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat ELISA, WB
SMAD3 Antibody LS-C117932 Rabbit Polyclonal thumb(1)
100 µg - 295.00
Applications: WB, ELISA Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat ELISA, WB
SMAD3 Antibody LS-C117930 Rabbit Polyclonal thumb(1)
100 µg - 295.00
Applications: WB, ELISA Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat ELISA, WB
SMAD3 Antibody LS-C60097 Rabbit Polyclonal thumb(1)
100 µg - 333.00
Applications: WB, ELISA Epitope: Internal  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human ELISA, WB
SMAD3 Antibody LS-C153854 Rabbit Polyclonal thumb(1)
100 µg - 380.00
Applications: WB, ELISA Epitope: pThr179  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human ELISA, WB
SMAD3 Antibody LS-C153867 Rabbit Polyclonal thumb(1)
100 µg - 380.00
Applications: WB, ELISA Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human WB
SMAD3 Antibody LS-C175877 Rabbit Polyclonal thumb(1)
100 µl - 345.00
Applications: WB Epitope: pThr179  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human WB
SMAD3 Antibody LS-C175878 Rabbit Polyclonal thumb(1)
100 µl - 345.00
Applications: WB Epitope: pSer213  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat WB
SMAD3 Antibody LS-C175902 Rabbit Polyclonal thumb(1)
100 µl - 345.00
Applications: WB Epitope: Met200  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat WB
SMAD3 Antibody LS-C152733 Rabbit Polyclonal thumb(1)
400 µg - 365.00
Applications: WB Epitope: aa206-220  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse WB
SMAD3 Antibody LS-C99969 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: Internal  
Reactivity: Hu, Mu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human DB
SMAD3 Antibody LS-C96970 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: DB Epitope: pSer208  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human ELISA, WB
SMAD3 Antibody LS-C46894 Rabbit Polyclonal
100 µg - 267.00
Applications: WB, ELISA Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human ELISA, WB
SMAD3 Antibody LS-C56835 Rabbit Polyclonal
50 µg - 320.00
Applications: WB, ELISA Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
SMAD3 Rabbit Monoclonal Human, Mouse, Rat ELISA, Flo, IF, IP, WB
SMAD3 Antibody LS-C39132 Rabbit Monoclonal
100 µl - 420.00
Applications: IF, WB, Flo... Epitope: N-Terminus  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse WB
SMAD3 Antibody LS-C10222 Rabbit Polyclonal
100 µl - 500.00
Applications: WB Epitope: pSer423/Ser425  
Reactivity: Hu, Mu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse, Rat WB
SMAD3 Antibody LS-C10219 Rabbit Polyclonal
400 µg - 445.00
Applications: WB Epitope: aa206-220  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human, Mouse WB
SMAD3 Antibody LS-C126302 Rabbit Polyclonal
100 µl - 415.00
Applications: WB Epitope: Ser423/Ser425  
Reactivity: Hu, Mu Format: Unconjugated  
SMAD3 Rabbit Polyclonal Human WB
SMAD3 Antibody LS-C132142 Rabbit Polyclonal
100 µl - 460.00
Applications: WB Epitope: aa122-365  
Reactivity: Hu Format: Unconjugated  

Immunohistochemistry

Immunohistochemistry
Anti-SMAD3 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2064 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 5/18/2013
Copyright © 2013 LifeSpan BioSciences, Inc. All Rights Reserved