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See All 29 RET Antibodies

Ordering
Wt. Vol. Conc. Price
500 µg 1000 µl 0.5 mg/ml $535

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

RET Antibody LS-C75422 Images

Flow Cytometry
Flow Cytometry Figure - Cell Count vs.

Anti-RET Antibody LS-C75422

Mouse anti-Human Monoclonal, Unconjugated

100% Guaranteed 100% Guaranteed

Details

Applications
Human RET
Mouse
Human, Mouse
IgG1 Monoclonal
Protein G Column
Unconjugated (Unconjugated)
Western blot (1 - 2 µg/ml)
Flo (50 µg/ml)
ELISA (0.5 - 1 µg/ml)
Optimal dilution to be determined by the researcher.

Specificity and Use

RET antibody was raised against sf21-derived rhRet/Fc Chimera.
Recognizes human Ret. Species cross-reactivity: Mouse.
Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 ug/ml; For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Western Blot: 1-2 ug/ml; Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 ug/ml; The detection limit for rhRet and rmRet is ~6 ng/well.

Packaging

Lyophilized from PBS, 5% trehalose.
1,000 µl Sterile PBS
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.

About RET

P07949 NM_020630 NP_065681.1

RET Antibody, Cadherin family member 12 Antibody, CDHF12 Antibody, C-ret Antibody, Hirschsprung disease 1 Antibody, HSCR1 Antibody, Hydroxyaryl-protein kinase Antibody, Oncogene ret Antibody, Proto-oncogene c-Ret Antibody, RET-ELE1 Antibody, RET51 Antibody, Ret proto-oncogene Antibody, RET transforming sequence Antibody, RET43 Antibody, Men2-ret Antibody, MEN2B Antibody, MTC1 Antibody, Receptor tyrosine kinase Antibody, CDHR16 Antibody, MEN2A Antibody, PTC Antibody, RET9 Antibody

The recombination of RET tyrosine kinase with several genes, such as GOLGA5 and the H4 gene, results in a consitutively active oncogenic protein.
(More About RET)

RET Antibodies (29)

Target Host Clonality Format Epitope Reactivity Applications
RET Rabbit Polyclonal Human IHC-P, WB
RET Antibody LS-B6923 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P, WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human ELISA, IHC-P, WB
RET Antibody [8D10C9] LS-B6328 Mouse Monoclonal thumb(2)
50 µl - 475.00
Applications: IHC-P, WB, ELISA Epitope: aa896-1063  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human IHC-P, WB
RET Antibody LS-B6584 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P, WB Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human IF, IHC, WB
RET Antibody LS-C162547 Mouse Monoclonal thumb(3)
100 µl - 290.00
Applications: IHC, IF, WB Epitope:  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human Flo, IHC-P, WB
RET Antibody LS-C99120 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC-P, WB, Flo Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Monoclonal Human, Mouse, Rat Flo, ICC, IHC-P, IP, WB
RET Antibody [EPR2871] LS-C138246 Rabbit Monoclonal thumb(2)
100 µl - 385.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
RET Mouse Monoclonal Human IHC-P
RET Antibody [3F8] LS-C87551 Mouse Monoclonal thumb(1)
0.1 ml - 260.00
0.5 ml - 470.00
1 ml - 610.00
Applications: IHC-P Epitope:  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human IHC-P, IP, WB
RET Antibody LS-C121292 Rabbit Polyclonal
100 µl - 415.00
Applications: IHC-P, WB, IP Epitope: pSer696  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human, Mouse, Rat ELISA, WB
RET Antibody LS-C117472 Rabbit Polyclonal thumb(1)
100 µg - 295.00
Applications: WB, ELISA Epitope: pTyr1062  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
RET Rabbit Polyclonal Human, Mouse, Rat ELISA, WB
RET Antibody LS-C118241 Rabbit Polyclonal thumb(1)
100 µg - 295.00
Applications: WB, ELISA Epitope: C-Terminus  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
RET Mouse Monoclonal Human ELISA, WB
RET Antibody LS-C41512 Mouse Monoclonal thumb(1)
100 µl - 322.00
Applications: WB, ELISA Epitope: aa896-1063  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human ELISA, WB
RET Antibody LS-C41514 Mouse Monoclonal thumb(1)
100 µl - 298.00
Applications: WB, ELISA Epitope: aa896-1063  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human ELISA, WB
RET Antibody [6E4C4] LS-C82173 Mouse Monoclonal thumb(1)
100 µg - 475.00
Applications: WB, ELISA Epitope: aa896-1063  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human ELISA, WB
RET Antibody [8D10C9] LS-C171386 Mouse Monoclonal thumb(1)
100 µl - 370.00
Applications: WB, ELISA Epitope: aa896-1063  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human ELISA, WB
RET Antibody [6E4C4] LS-C171391 Mouse Monoclonal thumb(1)
100 µl - 345.00
Applications: WB, ELISA Epitope: aa896-1063  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human, Mouse, Rat WB
RET Antibody LS-C177671 Rabbit Polyclonal thumb(1)
100 µl - 345.00
Applications: WB Epitope: Trp1056  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
RET Rabbit Polyclonal Human WB
RET Antibody LS-C155981 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope:  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human WB
RET Antibody LS-C99119 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
RET Goat Polyclonal Mouse ICC
RET Antibody LS-C87108 Goat Polyclonal
50 µg - 590.00
Applications: ICC Epitope: Extracellular Domain  
Reactivity: Mu Format: Unconjugated  
RET Rabbit Polyclonal Human ELISA
RET Antibody LS-C3184 Rabbit Polyclonal
100 µg - 270.00
Applications: ELISA Epitope: aa166-182  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human ELISA
RET Antibody LS-C3185 Rabbit Polyclonal
100 µg - 270.00
Applications: ELISA Epitope: aa1078-1093  
Reactivity: Hu Format: Unconjugated  
RET Rabbit Polyclonal Human WB
RET Antibody LS-C151180 Rabbit Polyclonal
50 µg - 380.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human
RET Antibody [RET01] LS-C95523 Mouse Monoclonal
0.5 ml - 335.00
1 ml - 455.00
Applications: Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human, Mouse ELISA, Flo, WB
RET Antibody LS-C75422 Mouse Monoclonal thumb(1)
500 µg - 535.00
Applications: WB, Flo, ELISA Epitope:  
Reactivity: Hu, Mu Format: Unconjugated  
RET Rabbit Polyclonal Human IP, WB
RET Antibody LS-C38815 Rabbit Polyclonal
100 µl - 430.00
Applications: WB, IP Epitope: pTyr905  
Reactivity: Hu Format: Unconjugated  
RET Mouse Monoclonal Human Flo
RET Antibody LS-C75423 Mouse Monoclonal
100 µg - 355.00
Applications: Flo Epitope:  
Reactivity: Hu Format: APC  
RET Mouse Monoclonal Human Flo
RET Antibody LS-C75424 Mouse Monoclonal
100 tst - 465.00
Applications: Flo Epitope:  
Reactivity: Hu Format: CF  
RET Mouse Monoclonal Human Flo
RET Antibody LS-C75425 Mouse Monoclonal
100 tst - 510.00
Applications: Flo Epitope:  
Reactivity: Hu Format: PE  
RET Rat Monoclonal Human, Mouse WB
RET Antibody LS-C90912 Rat Monoclonal
100 µg - 550.00
Applications: WB Epitope: Extracellular Domain  
Reactivity: Hu, Mu Format: Unconjugated  

Flow Cytometry

Flow Cytometry
Flow Cytometry Figure - Cell Count vs. Ret: SHSY-5Y human neuroblastoma cells were stained with LS-C75422 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.
  
Requested From: United States
Date Requested: 5/21/2013
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