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This antibody may not be available in some countries due to import restrictions. Please inquire before purchase.

Ordering

Wt. Vol. Conc. Price
100 µg - - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

PKM2 / Pyruvate Kinase Antibody LS-C150140 Images

Western Blot
Detection of Human, Mouse, and Rat PKM2 by Western Blot.
ICC
PKM2 in Wi-38 Human Cell Line.
ICC
PKM2 in RAW 264.7 Mouse Cell Line.
 

Anti-PKM2 / Pyruvate Kinase Antibody LS-C150140

Sheep anti-Human Polyclonal, Unconjugated

100% Guaranteed 100% Guaranteed

Details

Applications

Human PKM2 / Pyruvate Kinase
Sheep
Human, Mouse, Rat
IgG Polyclonal
Immunoaffinity Chromatography
Unconjugated (Unconjugated)
ICC (5 - 15 µg/ml)
Western blot (0.2 µg/ml)
Optimal dilution to be determined by the researcher.

Specificity and Use

PKM2 / Pyruvate Kinase antibody was raised against e. coli-derived recombinant human PKM2 Ser434-Pro531 Accession # P14618.
Detects human, mouse, rat PKM2 in Western blots and detects recombinant human PKM2 in direct ELISAs.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PKM2 / Pyruvate Kinase

P14618 NM_002654 NP_002645.3

PKM Antibody, p58 Antibody, PK3 Antibody, PKM2 Antibody, Pyruvate Kinase Antibody, Pyruvate kinase 2/3 Antibody, Pyruvate kinase, m1 Antibody, Pyruvate kinase, muscle Antibody, TCB Antibody, Tumor M2-PK Antibody, OIP-3 Antibody, OIP3 Antibody, Opa-interacting protein 3 Antibody, CTHBP Antibody, PK, muscle type Antibody, PK2 Antibody, Pyruvate kinase isozymes M1/M2 Antibody, Pyruvate kinase muscle isozyme Antibody, THBP1 Antibody

This gene encodes a protein involved in glycolysis.


PKM2 / Pyruvate Kinase Antibodies (33)

Target Host Clonality Format Epitope Reactivity Applications
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit ELISA, IHC-P, IP, WB
PKM2 / Pyruvate Kinase Antibody LS-B1450 Goat Polyclonal thumb(1)
50 µl - 385.00
Applications: IHC-P, WB, IP... Epitope:  
Reactivity: Rb Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Monkey, Mouse, Rat IHC-Fr, IHC-P, WB
PKM2 / Pyruvate Kinase Antibody LS-B2445 Rabbit Polyclonal thumb(1)
50 µl - 385.00
Applications: IHC-Fr, IHC-P, WB Epitope:  
Reactivity: Hu, Mo, Mu ... Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human Flo, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C163750 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC, WB, Flo Epitope: aa123-153  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Bovine, Human, Mouse, Rat ICC, IF, IHC-P, WB
PKM2 / Pyruvate Kinase Antibody LS-C143414 Rabbit Polyclonal thumb(3)
100 µl - 330.00
Applications: IHC-P, ICC, IF... Epitope: aa350-450  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human Flo, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C163751 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC, WB, Flo Epitope: aa393-422  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Mouse IHC-P, WB
PKM2 / Pyruvate Kinase Antibody LS-C100685 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC-P, WB Epitope: C-Terminus  
Reactivity: Hu, Mu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Mouse IHC-P, WB
PKM2 / Pyruvate Kinase Antibody LS-C181996 Rabbit Polyclonal thumb(2)
100 µl - 335.00
Applications: IHC-P, WB Epitope:  
Reactivity: Hu, Mu Format: Unconjugated  
PKM2 / Pyruvate Ki... Sheep Polyclonal Rabbit ELISA, IF, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C147646 Sheep Polyclonal
10000 µg - 195.00
Applications: IHC, IF, WB... Epitope:  
Reactivity: Rb Format: Unconjugated  
PKM2 / Pyruvate Ki... Sheep Polyclonal Rabbit ELISA, IF, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C147647 Sheep Polyclonal
1000 µl - 330.00
Applications: IHC, IF, WB... Epitope:  
Reactivity: Rb Format: Biotin  
PKM2 / Pyruvate Ki... Sheep Polyclonal Rabbit ELISA, IF, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C147648 Sheep Polyclonal
1000 µg - 390.00
Applications: IHC, IF, WB... Epitope:  
Reactivity: Rb Format: Unconjugated  
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit ELISA, IF, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C59511 Goat Polyclonal
10000 µg - 333.00
Applications: IHC, IF, WB... Epitope:  
Reactivity: Rb Format: Biotin  
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit ELISA, IHC, WB
PKM2 / Pyruvate Kinase Antibody LS-C59503 Goat Polyclonal
20000 µg - 333.00
Applications: IHC, WB, ELISA Epitope:  
Reactivity: Rb Format: HRP  
PKM2 / Pyruvate Ki... Sheep Polyclonal Human, Mouse, Rat ICC, WB
PKM2 / Pyruvate Kinase Antibody LS-C150140 Sheep Polyclonal thumb(3)
100 µg - 395.00
Applications: ICC, WB Epitope:  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Mouse, Rat WB
PKM2 / Pyruvate Kinase Antibody LS-C178252 Rabbit Polyclonal thumb(1)
100 µl - 345.00
Applications: WB Epitope: Lys230  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Mouse WB
PKM2 / Pyruvate Kinase Antibody LS-C98972 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: N-Terminus  
Reactivity: Hu, Mu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Mouse, Primate, Rat WB
PKM2 / Pyruvate Kinase Antibody LS-C98973 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu, Mu, Pr ... Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human, Mouse WB
PKM2 / Pyruvate Kinase Antibody LS-C99052 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu, Mu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human WB
PKM2 / Pyruvate Kinase Antibody LS-C99053 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human WB
PKM2 / Pyruvate Kinase Antibody LS-C117034 Rabbit Polyclonal thumb(1)
50 µg - 380.00
Applications: WB Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human WB
PKM2 / Pyruvate Kinase Antibody LS-C117151 Rabbit Polyclonal thumb(1)
50 µg - 380.00
Applications: WB Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit ELISA, IP, WB
PKM2 / Pyruvate Kinase Antibody LS-C59237 Goat Polyclonal
2000 µl - 370.00
Applications: WB, IP, ELISA Epitope:  
Reactivity: Rb Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Bovine, Human, Mouse, Rat ICC, WB
PKM2 / Pyruvate Kinase Antibody LS-C143415 Rabbit Polyclonal
100 µl - 330.00
Applications: ICC, WB Epitope: aa350-450  
Reactivity: Bo, Hu, Mu ... Format: Biotin  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Bovine, Human, Mouse, Rat ICC, WB
PKM2 / Pyruvate Kinase Antibody LS-C143416 Rabbit Polyclonal
100 µl - 330.00
Applications: ICC, WB Epitope: aa350-450  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Bovine, Human, Mouse, Rat ICC, WB
PKM2 / Pyruvate Kinase Antibody LS-C143417 Rabbit Polyclonal
100 µl - 330.00
Applications: ICC, WB Epitope: aa350-450  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Bovine, Human, Mouse, Rat ICC, WB
PKM2 / Pyruvate Kinase Antibody LS-C143418 Rabbit Polyclonal
100 µl - 330.00
Applications: ICC, WB Epitope: aa350-450  
Reactivity: Bo, Hu, Mu ... Format: HRP  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Bovine, Human, Mouse, Rat ICC, WB
PKM2 / Pyruvate Kinase Antibody LS-C143419 Rabbit Polyclonal
100 µl - 330.00
Applications: ICC, WB Epitope: aa350-450  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
PKM2 / Pyruvate Ki... Mouse Monoclonal Human
PKM2 / Pyruvate Kinase Antibody LS-C113047 Mouse Monoclonal
50 µl - 225.00
Applications: Epitope:  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit
PKM2 / Pyruvate Kinase Antibody LS-C86196 Goat Polyclonal
50000 µg - 500.00
Applications: Epitope:  
Reactivity: Rb Format: Unconjugated  
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit ELISA, IF, IP, WB
PKM2 / Pyruvate Kinase Antibody LS-C38680 Goat Polyclonal
1000 µg - 230.00
Applications: IF, WB, IP... Epitope:  
Reactivity: Rb Format: Biotin  
PKM2 / Pyruvate Ki... Goat Polyclonal Rabbit ELISA, IP, WB
PKM2 / Pyruvate Kinase Antibody LS-C38678 Goat Polyclonal
1000 µl - 280.00
Applications: WB, IP, ELISA Epitope:  
Reactivity: Rb Format: Unconjugated  
PKM2 / Pyruvate Ki... Mouse Monoclonal Human ELISA, IF, WB
PKM2 / Pyruvate Kinase Antibody LS-C131760 Mouse Monoclonal
100 µl - 425.00
Applications: IF, WB, ELISA Epitope: aa1-531  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Rabbit Polyclonal Human ELISA, WB
PKM2 / Pyruvate Kinase Antibody LS-C131761 Rabbit Polyclonal
100 µg - 525.00
Applications: WB, ELISA Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
PKM2 / Pyruvate Ki... Sheep Polyclonal Human ELISA
PKM2 / Pyruvate Kinase Antibody LS-C93516 Sheep Polyclonal
1000 µl - 530.00
Applications: ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  

Western Blot

Western Blot
Detection of Human, Mouse, and Rat PKM2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 ug/ml of Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for PKM2 at approximately 60 kD (as indicated). This experiment was conducted under reducing conditions.

ICC

ICC
PKM2 in Wi-38 Human Cell Line. PKM2 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

ICC

ICC
PKM2 in RAW 264.7 Mouse Cell Line. PKM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
 

Requested From: United States
Date Requested: 5/24/2013
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