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Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $385

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OLFM4 Antibody LS-B2055 Images

Immunohistochemistry
Anti-OLFM4 antibody IHC staining of human colon.
Western Blot
Western blot analysis of human Olfactomedin 4 in human liver lysate in the 1) absence and 2)...

Anti-OLFM4 Antibody LS-B2055 IHC-plus™

Rabbit anti-Human Polyclonal, aa400-450, Unconjugated

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Details

Applications

Human OLFM4
Rabbit
Human, Bovine, Chimpanzee, Dog, Monkey, Mouse, Rat
Gibbon, Marmoset, Gorilla
Polyclonal
Immunoaffinity Chromatography
Unconjugated (Unconjugated)
IHC-P (5 µg/ml)
Western blot (0.5 - 2 µg/ml)
Optimal dilution to be determined by the researcher.

Specificity and Use

OLFM4 antibody was raised against a synthetic peptide corresponding to amino acids 400-419 (YVYNDGYLLNYDLSVLQKPQ) of human OLFM4 was used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Marmoset (95%); Monkey, Mouse, Rat (85%).
aa400-450
A portion of amino acids 400-450 of human Olfactomedin 4
Immunohistochemistry: LS-B2055 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2055 was determined to be 5 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About OLFM4

NM_006418 NP_006409.3

OLFM4 Antibody, Antiapoptotic protein GW112 Antibody, HOLfD Antibody, HGC-1 Antibody, GW112 Antibody, OlfD Antibody, OLM4 Antibody, UNQ362 Antibody, Olfactomedin 4 Antibody, Olfactomedin-4 Antibody, BA209J19.1 Antibody, GC1 Antibody, HCG-1 Antibody

Olfactomedin 4 (OLFM4) was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium.


OLFM4 Antibodies (6)

Target Host Clonality Format Epitope Reactivity Applications
OLFM4 Rabbit Polyclonal Human IHC-P
OLFM4 Antibody LS-A8455 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
OLFM4 Rabbit Polyclonal Human IHC-P
OLFM4 Antibody LS-A9926 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
OLFM4 Rabbit Polyclonal Human IHC-P
OLFM4 Antibody LS-A8454 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
OLFM4 Rabbit Polyclonal Bovine, Chimpanzee, Dog, Human, Monkey, Mouse, Rat IHC-P, WB
OLFM4 Antibody LS-B2055 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P, WB Epitope: aa400-450  
Reactivity: Bo, Ch, Do ... Format: Unconjugated  
OLFM4 Rabbit Polyclonal Human ELISA, WB
OLFM4 Antibody LS-C47070 Rabbit Polyclonal
100 µg - 267.00
Applications: WB, ELISA Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
OLFM4 Rabbit Polyclonal Bovine, Dog, Human WB
OLFM4 Antibody LS-C81617 Rabbit Polyclonal thumb(1)
100 µg - 335.00
Applications: WB Epitope: C-Terminus  
Reactivity: Bo, Do, Hu Format: Unconjugated  

Immunohistochemistry

Immunohistochemistry
Anti-OLFM4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2055 concentration 5 ug/ml.

Western Blot

Western Blot
Western blot analysis of human Olfactomedin 4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using LS-B2055 at 2 ug/ml.

Requested From: United States
Date Requested: 5/21/2013
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