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The Immunohistochemistry Antibody Company
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Ordering |
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LSBio (Direct) |
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Anti-CD223 / LAG-3 Antibody LS-C44775
Rat anti-Mouse Monoclonal, Clone C9B7W, RPE
100% Guaranteed
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Details |
Applications |
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Specificity and Use
| CD223 / LAG-3 antibody was raised against murine CD223 Ig fusion protein. | |
| Recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Recognizes an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. XXX recommend the use of MCA2386EL for this purpose. | |
| Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. |
Packaging
| Lyophilized, PBS, pH 7.4, 0.09% sodium azide, 1% BSA. | |
| 100 tst Distilled Water. | |
| For research use only. |
About CD223 / LAG-3
P18627
NM_002286
LAG3 Antibody, CD223 antigen Antibody, LAG-3 Antibody, Protein FDC Antibody, CD223 Antibody, FDC Antibody, Lymphocyte-activation gene 3 Antibody
Lymphocyte-Activation Protein 3 (LAG3) belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons.
PDF Spec Sheet
Technical HelpCD223 / LAG-3 Antibodies (12)
| Target | Host | Clonality | Format | Epitope | Reactivity | Applications | |||||||||||
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| CD223 / LAG-3 | Mouse | Monoclonal | Human | Flo, IHC-P |
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| CD223 / LAG-3 | Mouse | Monoclonal | Human | ICC, WB |
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| CD223 / LAG-3 | Mouse | Monoclonal | Human | ELISA |
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| CD223 / LAG-3 | Rabbit | Polyclonal | Human | IHC, WB |
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| CD223 / LAG-3 | Mouse | Monoclonal | Human | ELISA, ICC, IHC-Fr, IP, WB |
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| CD223 / LAG-3 | Rabbit | Polyclonal | Human | WB |
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| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo, Func |
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| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo |
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| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo |
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| CD223 / LAG-3 | Rat | Monoclonal | Mouse | Flo, Func |
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| CD223 / LAG-3 | Goat | Polyclonal | Human | Flo |
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| CD223 / LAG-3 | Goat | Polyclonal | Mouse | Flo |
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Requested From: United States
Date Requested: 5/25/2013
Date Requested: 5/25/2013