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See All 133 HIF1A / HIF1 Alpha Antibodies

Ordering
Wt. Vol. Conc. Price
- 100 µl - $335

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

HIF1A / HIF1 Alpha Antibody LS-C83705 Images

Immunohistochemistry
Staining of HIF-1 alpha in human kidney.
Western Blot
On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells).
Western Blot
Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts.
Immunoprecipitation
Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected.

Anti-HIF1A / HIF1 Alpha Antibody LS-C83705

Mouse anti-Human Monoclonal, aa432-528, Clone H1alpha67, HRP

100% Guaranteed 100% Guaranteed

Details

Applications
Human HIF1A / HIF1 Alpha
Mouse
Human, Bovine, Ferret, Mouse, Pig, Primate, Rat, Sheep
IgG2b Monoclonal [H1alpha67]
Protein G Column
Horseradish Peroxidase (HRP)
IHC (1:500 - 1:1000)
Western blot
Optimal dilution to be determined by the researcher.

Specificity and Use

HIF1A / HIF1 Alpha antibody was raised against fusion protein containing amino acids 432-528 of human HIF-1alpha
aa432-528
HIF-1 alpha.
By Western blot, recognizes bands at 120kD representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120kD representing post-translational modification. Can also be used for immunohistochemical detection of human HIF-1 alpha and immunoprecipitation. ** Nuclear Extracts.

Packaging

Phosphate buffered saline
Store at 4°C. Do not freeze.
For research use only.

About HIF1A / HIF1 Alpha

Q16665 NM_001530 NP_001521.1

HIF1A Antibody, ARNT-interacting protein Antibody, ARNT interacting protein Antibody, BHLHe78 Antibody, HIF1 Alpha Antibody, Hypoxia-inducible factor1alpha Antibody, HIF-1A Antibody, HIF1 Antibody, MOP1 Antibody, Member of PAS superfamily 1 Antibody, PASD8 Antibody, HIF-1-alpha Antibody, HIF-1alpha Antibody, HIF1-ALPHA Antibody, Member of PAS protein 1 Antibody

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential rol.
(More About HIF1A / HIF1 Alpha)

HIF1A / HIF1 Alpha Antibodies (133)

Target Host Clonality Format Epitope Reactivity Applications
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-B495 Rabbit Polyclonal thumb(1)
100 µl - 385.00
Applications: IHC-P, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Guinea pig, Human, Mouse, Primate, Rat IHC, IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-B2823 Rabbit Polyclonal thumb(3)
100 µl - 385.00
Applications: IHC, IHC-P, WB... Epitope: aa432-528  
Reactivity: Gp, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Mouse, Pig IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-B674 Rabbit Polyclonal thumb(2)
100 µl - 385.00
Applications: IHC-P, WB Epitope: aa432-528  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rabbit, Rat, Sheep IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-B110 Mouse Monoclonal thumb(1)
50 µl - 385.00
Applications: IHC-P, WB, IP Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-B4816 Rabbit Polyclonal thumb(2)
50 µl - 385.00
Applications: IHC-P, WB, IP Epitope: aa609-826  
Reactivity: Hu, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Monkey, Mouse IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-B1503 Rabbit Polyclonal thumb(2)
125 µl - 385.00
Applications: IHC-P, WB Epitope: C-Terminus  
Reactivity: Hu, Mo, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Dog, Human, Mouse, Rat ICC, IF, IHC-P
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-B109 Mouse Monoclonal thumb(1)
50 µl - 385.00
Applications: IHC-P, ICC, IF Epitope: aa329-530  
Reactivity: Bo, Do, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Chicken, Human, Mouse, Pig, Rabbit, Rat, Xenopus IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-B5879 Rabbit Polyclonal thumb(1)
50 µg - 385.00
Applications: IHC-P, WB Epitope: N-Terminus  
Reactivity: Bo, Ck, Fr ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human IHC-P, WB
HIF1A / HIF1 Alpha Antibody [HA111] LS-B145 Mouse Monoclonal thumb(1)
100 µl - 385.00
Applications: IHC-P, WB Epitope: aa329-530  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Rat IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-B6182 Rabbit Polyclonal thumb(1)
50 µl - 385.00
Applications: IHC-P, WB, IP Epitope:  
Reactivity: Hu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse Flo, IF, IHC, WB
HIF1A / HIF1 Alpha Antibody LS-C166867 Rabbit Polyclonal thumb(4)
100 µg - 290.00
Applications: IHC, IF, WB... Epitope: aa520-550  
Reactivity: Hu, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Monoclonal Human, Rat ICC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [EP1215Y] LS-C49968 Rabbit Monoclonal thumb(2)
100 µl - 415.00
Applications: IHC-P, ICC, WB Epitope: C-Terminus  
Reactivity: Hu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC, WB
HIF1A / HIF1 Alpha Antibody LS-C83732 Rabbit Polyclonal
100 µl - 335.00
Applications: IHC, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Monkey, Mouse ELISA, ICC, IHC, WB
HIF1A / HIF1 Alpha Antibody [1A3] LS-C169345 Mouse Monoclonal thumb(5)
100 µl - 350.00
Applications: IHC, ICC, WB... Epitope:  
Reactivity: Hu, Mo, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rabbit, Rat, Sheep IF, IHC, IHC-Fr, IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140776 Mouse Monoclonal thumb(1)
250 µl - 330.00
Applications: IHC, IHC-Fr, IF... Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: Biotin  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C83705 Mouse Monoclonal thumb(4)
100 µl - 335.00
Applications: IHC, WB Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Monkey, Mouse ELISA, IHC, WB
HIF1A / HIF1 Alpha Antibody [1A3] LS-C171583 Mouse Monoclonal thumb(3)
100 µl - 365.00
Applications: IHC, WB, ELISA Epitope:  
Reactivity: Hu, Mo, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Hamster, Human IF, IHC, WB
HIF1A / HIF1 Alpha Antibody LS-C166866 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC, IF, WB Epitope: aa1-31  
Reactivity: Ha, Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human Flo, IHC, WB
HIF1A / HIF1 Alpha Antibody LS-C166864 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IHC, WB, Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Dog, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C2557 Mouse Monoclonal thumb(2)
100 µl - 335.00
Applications: IHC, WB, IP Epitope: aa432-528  
Reactivity: Bo, Do, Fe ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Rat IHC, WB
HIF1A / HIF1 Alpha Antibody LS-C177574 Rabbit Polyclonal thumb(2)
100 µl - 345.00
Applications: IHC, WB Epitope: Lys377  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ICC, IF, IHC, IP
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C83706 Mouse Monoclonal thumb(1)
100 µl - 335.00
Applications: IHC, ICC, IF... Epitope: aa329-530  
Reactivity: Bo, Hu, Mu ... Format: Biotin  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ICC, IF, IHC, IHC-Fr, IHC-P, IP
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C140822 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa329-530  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ICC, IF, IHC, IHC-Fr, IHC-P, IP
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C140823 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa329-530  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ICC, IF, IHC, IHC-Fr, IHC-P, IP
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C140824 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, IHC-Fr... Epitope: aa329-530  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ELISA, ICC, IF, IHC-Fr, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C150386 Mouse Monoclonal
25 µg - 175.00
Applications: IHC-Fr, IHC-P, ICC... Epitope:  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rabbit, Rat, Sheep IF, IHC, IHC-Fr, IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140777 Mouse Monoclonal
250 µl - 330.00
Applications: IHC, IHC-Fr, IF... Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: DY650  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rabbit, Rat, Sheep IF, IHC, IHC-Fr, IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140778 Mouse Monoclonal
250 µl - 330.00
Applications: IHC, IHC-Fr, IF... Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: DY488  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rabbit, Rat, Sheep IF, IHC, IHC-Fr, IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140780 Mouse Monoclonal
250 µl - 330.00
Applications: IHC, IHC-Fr, IF... Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: DY550  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ICC, IHC-Fr, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C153079 Mouse Monoclonal
100 µg - 335.00
Applications: IHC-Fr, IHC-P, ICC... Epitope:  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-C141170 Rabbit Polyclonal
100 µl - 330.00
Applications: IHC, IHC-P, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: DY650  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140802 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, WB Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: Biotin  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140803 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, WB Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140804 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, WB Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140805 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, WB Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: HiLyte Fluor, HiLyte Fluor 488  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rat, Sheep IHC, IHC-P, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140806 Mouse Monoclonal
100 µl - 330.00
Applications: IHC, IHC-P, WB Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Mouse, Rat IHC-Fr, IHC-P
HIF1A / HIF1 Alpha Antibody LS-C7465 Mouse Monoclonal
100 µl - 460.00
Applications: IHC-Fr, IHC-P Epitope: aa329-530  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human IHC
HIF1A / HIF1 Alpha Antibody LS-C96684 Rabbit Polyclonal thumb(2)
100 µg - 290.00
Applications: IHC Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Guinea pig, Human, Mouse, Pig, Primate, Rat GS, ICC, IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C181014 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Gp, Hu, Mu ... Format: DY550  
HIF1A / HIF1 Alpha Rabbit Polyclonal Guinea pig, Human, Mouse, Pig, Primate, Rat GS, ICC, IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C181016 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Gp, Hu, Mu ... Format: Biotin  
HIF1A / HIF1 Alpha Rabbit Polyclonal Guinea pig, Human, Mouse, Pig, Primate, Rat GS, ICC, IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C181017 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Gp, Hu, Mu ... Format: DY650  
HIF1A / HIF1 Alpha Rabbit Polyclonal Guinea pig, Human, Mouse, Pig, Primate, Rat GS, ICC, IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C181018 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Gp, Hu, Mu ... Format: DY488  
HIF1A / HIF1 Alpha Rabbit Polyclonal Guinea pig, Human, Mouse, Pig, Primate, Rat GS, ICC, IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C181019 Rabbit Polyclonal
100 µl - 365.00
Applications: IHC-P, ICC, WB... Epitope:  
Reactivity: Gp, Hu, Mu ... Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat, Sheep IHC, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C26700 Mouse Monoclonal
100 µg - 470.00
Applications: IHC, WB, IP Epitope: aa432-528  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-C141171 Rabbit Polyclonal
100 µl - 330.00
Applications: IHC-P, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: DY488  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-C141172 Rabbit Polyclonal
100 µl - 330.00
Applications: IHC-P, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: DY550  
HIF1A / HIF1 Alpha Mouse Monoclonal Ferret, Human, Mouse, Rat, Sheep IHC-P
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C87583 Mouse Monoclonal
20 µg - 270.00
100 µg - 435.00
1 ml - 620.00
Applications: IHC-P Epitope:  
Reactivity: Fe, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Ferret, Human, Mouse, Rat, Sheep IHC-P
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C87584 Mouse Monoclonal
100 µg - 465.00
200 µg - 665.00
Applications: IHC-P Epitope:  
Reactivity: Fe, Hu, Mu ... Format: Azide-free  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse IHC, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C7453 Rabbit Polyclonal
50 µg - 422.00
Applications: IHC, WB, IP Epitope: aa609-826  
Reactivity: Hu, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Pig, Rat, Sheep IHC-P, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C7464 Mouse Monoclonal
100 µl - 460.00
Applications: IHC-P, WB, IP Epitope: aa432-528  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Rat IHC, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C123751 Rabbit Polyclonal
100 µg - 395.00
Applications: IHC, WB, IP Epitope: C-Terminus  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Monkey, Mouse Flo, IHC, WB
HIF1A / HIF1 Alpha Antibody LS-C123797 Rabbit Polyclonal
20 µg - 465.00
Applications: IHC, WB, Flo Epitope: C-Terminus  
Reactivity: Hu, Mo, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-C121233 Rabbit Polyclonal
100 µl - 445.00
Applications: IHC-P, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: HRP  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Primate, Rat IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-C121234 Rabbit Polyclonal
100 µg - 445.00
Applications: IHC-P, WB Epitope: aa530-825  
Reactivity: Hu, Mu, Pr ... Format: Biotin  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Rabbit, Rat IHC-P, WB
HIF1A / HIF1 Alpha Antibody LS-C121964 Rabbit Polyclonal
100 µg - 440.00
Applications: IHC-P, WB Epitope: aa775-790  
Reactivity: Hu, Mu, Rb ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human Flo, IF, WB
HIF1A / HIF1 Alpha Antibody LS-C166865 Rabbit Polyclonal thumb(3)
100 µg - 290.00
Applications: IF, WB, Flo Epitope: aa710-740  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human DB, Flo
HIF1A / HIF1 Alpha Antibody LS-C165096 Rabbit Polyclonal thumb(2)
100 µg - 290.00
Applications: Flo, DB Epitope: aa547-577  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Dog, Human, Monkey, Mouse, Rat, Xenopus ELISA, WB
HIF1A / HIF1 Alpha Antibody LS-C59282 Rabbit Polyclonal thumb(1)
100 µl - 350.00
Applications: WB, ELISA Epitope: Hydroxy-Pro564  
Reactivity: Bo, Do, Fr ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Mouse, Pig, Primate, Rabbit, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C83866 Rabbit Polyclonal thumb(1)
100 µl - 335.00
Applications: WB Epitope: aa400-550  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Chicken, Dog, Human, Mouse, Pig, Rabbit, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C110238 Rabbit Polyclonal thumb(1)
50 µg - 370.00
Applications: WB Epitope: Internal  
Reactivity: Bo, Ck, Do ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C134910 Rabbit Polyclonal thumb(1)
50 µg - 380.00
Applications: WB Epitope: N-Terminus  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C171749 Rabbit Polyclonal thumb(1)
100 µg - 260.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu, Mu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C157806 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: aa735-762  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C159833 Mouse Monoclonal thumb(1)
100 µl - 290.00
Applications: WB Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Mouse, Pig, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C143395 Rabbit Polyclonal thumb(1)
100 µl - 335.00
Applications: WB Epitope: Exon 12  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Chicken, Human, Mouse, Primate, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C143401 Rabbit Polyclonal thumb(1)
100 µl - 335.00
Applications: WB Epitope: Exon 13  
Reactivity: Bo, Ck, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Mouse, Pig, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C143383 Rabbit Polyclonal thumb(1)
100 µl - 335.00
Applications: WB Epitope: Exon 9  
Reactivity: Bo, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Pig WB
HIF1A / HIF1 Alpha Antibody LS-C143389 Rabbit Polyclonal thumb(1)
100 µl - 335.00
Applications: WB Epitope: Exon 10  
Reactivity: Bo, Hu, Po Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Human, Mouse, Rat ICC, IF, IP
HIF1A / HIF1 Alpha Antibody [ESEE122] LS-C83707 Mouse Monoclonal
100 µl - 335.00
Applications: ICC, IF, IP Epitope: aa329-530  
Reactivity: Bo, Hu, Mu ... Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Human GS, IF, IP
HIF1A / HIF1 Alpha Antibody LS-C26699 Mouse Monoclonal
100 µg - 470.00
Applications: IF, IP, GS Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human GS, IF
HIF1A / HIF1 Alpha Antibody [SPM404] LS-C33869 Mouse Monoclonal
50 µg - 295.00
Applications: IF, GS Epitope: aa530-826  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human GS, IF
HIF1A / HIF1 Alpha Antibody [OZ12] LS-C88380 Mouse Monoclonal
20 µg - 250.00
0.5 ml - 425.00
200 µg - 560.00
Applications: IF, GS Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human GS, IF
HIF1A / HIF1 Alpha Antibody [OZ12] LS-C88381 Mouse Monoclonal
100 µg - 455.00
200 µg - 595.00
Applications: IF, GS Epitope:  
Reactivity: Hu Format: Azide-free  
HIF1A / HIF1 Alpha Mouse Monoclonal Bovine, Ferret, Human, Mouse, Pig, Primate, Rabbit, Rat, Sheep IP, WB
HIF1A / HIF1 Alpha Antibody [H1alpha67] LS-C140779 Mouse Monoclonal
250 µl - 330.00
Applications: WB, IP Epitope: aa432-528  
Reactivity: Bo, Fe, Hu ... Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [HA111] LS-C141084 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa329-530  
Reactivity: Hu Format: Biotin  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [HA111] LS-C141085 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa329-530  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [HA111] LS-C141086 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa329-530  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [HA111] LS-C141087 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa329-530  
Reactivity: Hu Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [H1alpha 67-7] LS-C140817 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu Format: Biotin  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [H1alpha 67-7] LS-C140818 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [H1alpha 67-7] LS-C140819 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [H1alpha 67-7] LS-C140820 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu Format: HRP  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [H1alpha 67-7] LS-C140821 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody [HA111] LS-C140760 Mouse Monoclonal
100 µl - 330.00
Applications: WB Epitope: aa329-530  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C94217 Rabbit Polyclonal
500 µg - 343.00
Applications: WB Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Primate WB
HIF1A / HIF1 Alpha Antibody [H1alpha 67-7] LS-C2554 Mouse Monoclonal
100 µl - 335.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu, Pr Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143390 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-450  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143391 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-450  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143392 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-450  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143393 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-450  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143394 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-450  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143384 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa350-400  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143385 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa350-400  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143386 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa350-400  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143387 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa350-400  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143388 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa350-400  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Pig WB
HIF1A / HIF1 Alpha Antibody LS-C141259 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Bo, Hu, Po Format: Biotin  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Pig WB
HIF1A / HIF1 Alpha Antibody LS-C141260 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Bo, Hu, Po Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Pig WB
HIF1A / HIF1 Alpha Antibody LS-C141261 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Bo, Hu, Po Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Pig WB
HIF1A / HIF1 Alpha Antibody LS-C141262 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Bo, Hu, Po Format: HRP  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Human, Pig WB
HIF1A / HIF1 Alpha Antibody LS-C141263 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa432-528  
Reactivity: Bo, Hu, Po Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Dog, Human, Mouse, Pig, Primate, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C142696 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-550  
Reactivity: Bo, Do, Hu ... Format: Biotin  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Dog, Human, Mouse, Pig, Primate, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C142697 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-550  
Reactivity: Bo, Do, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Dog, Human, Mouse, Pig, Primate, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C142698 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-550  
Reactivity: Bo, Do, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Dog, Human, Mouse, Pig, Primate, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C142699 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-550  
Reactivity: Bo, Do, Hu ... Format: HRP  
HIF1A / HIF1 Alpha Rabbit Polyclonal Bovine, Dog, Human, Mouse, Pig, Primate, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C142700 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa400-550  
Reactivity: Bo, Do, Hu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143402 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa700-750  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143403 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa700-750  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143404 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa700-750  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143405 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa700-750  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143406 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa700-750  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143396 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa550-600  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143397 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa550-600  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143398 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa550-600  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143399 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa550-600  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C143400 Rabbit Polyclonal
100 µl - 330.00
Applications: WB Epitope: aa550-600  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C144839 Rabbit Polyclonal
100 µl - 355.00
Applications: WB Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C144840 Rabbit Polyclonal
100 µl - 355.00
Applications: WB Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Rat IP
HIF1A / HIF1 Alpha Antibody LS-C171750 Rabbit Polyclonal
100 µg - 325.00
Applications: IP Epitope: C-Terminus  
Reactivity: Hu, Mu, Rt Format: Magnetic beads  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Mouse, Rat IP
HIF1A / HIF1 Alpha Antibody LS-C171751 Rabbit Polyclonal
100 µg - 325.00
Applications: IP Epitope: C-Terminus  
Reactivity: Hu, Mu, Rt Format: Sepharose  
HIF1A / HIF1 Alpha Mouse Monoclonal Human
HIF1A / HIF1 Alpha Antibody [OZ12] LS-C95509 Mouse Monoclonal
0.5 ml - 315.00
1 ml - 420.00
Applications: Epitope: aa530-826  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Mouse GS, ICC, IP, WB
HIF1A / HIF1 Alpha Antibody LS-C7454 Mouse Monoclonal
100 µl - 580.00
Applications: ICC, WB, IP... Epitope: aa530-826  
Reactivity: Hu, Mu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human GS, IF, WB
HIF1A / HIF1 Alpha Antibody LS-C7457 Mouse Monoclonal
250 µl - 330.00
Applications: IF, WB, GS Epitope: aa530-826  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Ferret, Human, Mouse, Rat, Sheep IP, WB
HIF1A / HIF1 Alpha Antibody LS-C7469 Mouse Monoclonal
100 µg - 455.00
Applications: WB, IP Epitope: aa432-528  
Reactivity: Fe, Hu, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Mouse, Rat, Sheep ELISA, WB
HIF1A / HIF1 Alpha Antibody LS-C36834 Mouse Monoclonal
50 µg - 450.00
Applications: WB, ELISA Epitope: aa432-528  
Reactivity: Hu, Mu, Rt ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human ELISA, WB
HIF1A / HIF1 Alpha Antibody LS-C36835 Mouse Monoclonal
100 µg - 550.00
Applications: WB, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human ELISA, WB
HIF1A / HIF1 Alpha Antibody LS-C36836 Mouse Monoclonal
100 µg - 550.00
Applications: WB, ELISA Epitope: aa530-826  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human GS, IF
HIF1A / HIF1 Alpha Antibody LS-C124955 Mouse Monoclonal
500 µl - 480.00
Applications: IF, GS Epitope: aa530-826  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human, Rat WB
HIF1A / HIF1 Alpha Antibody LS-C124956 Rabbit Polyclonal
100 µl - 425.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu, Rt Format: Unconjugated  
HIF1A / HIF1 Alpha Rabbit Polyclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C124952 Rabbit Polyclonal
100 µl - 425.00
Applications: WB Epitope: aa400-550  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C124953 Mouse Monoclonal
100 µl - 420.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human, Monkey, Mouse, Rat, Sheep WB
HIF1A / HIF1 Alpha Antibody LS-C7463 Mouse Monoclonal
250 µl - 460.00
Applications: WB Epitope: aa432-528  
Reactivity: Hu, Mo, Mu ... Format: Unconjugated  
HIF1A / HIF1 Alpha Mouse Monoclonal Human WB
HIF1A / HIF1 Alpha Antibody LS-C7455 Mouse Monoclonal
100 µg - 445.00
Applications: WB Epitope: aa329-530  
Reactivity: Hu Format: Unconjugated  

Immunohistochemistry

Immunohistochemistry
Staining of HIF-1 alpha in human kidney.

Western Blot

Western Blot
On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). -On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease inhibitors. Upon quantification, 100 ug of protein was fractionated on 7% polyacrylamide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution.

Western Blot

Western Blot
Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected.
Requested From: United States
Date Requested: 5/18/2013
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