|
The Immunohistochemistry Antibody Company
|
|
|
Ordering |
|
|
LSBio (Direct) |
|
|
DNMT3B Antibody LS-B1191 Images
Anti-DNMT3B antibody IHC staining of human melanoma.
|
Anti-DNMT3B Antibody LS-B1191 IHC-plus™
Rabbit Polyclonal, aa4-101, Unconjugated
100% Guaranteed
|
Publications (11)
|
Details |
Applications |
||||||||||||||||
|
|
Specificity and Use
| DNMT3B antibody was raised against this antibody was raised against residues 4-101 of the human DNMT3b protein | |
| aa4-101 | |
| DNMT3b. | |
| Immunohistochemistry: LS-B1191 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1191 was determined to be 1:200. |
Packaging
| No preservative. | |
| Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. | |
| For research use only. |
Publications (11)
1
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B. Nature. 2002 416:552-6. (WB; Human)
[PubMed:11932749]
2
Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells. Weisenberger DJ, Velicescu M, Preciado-Lopez MA, Gonzales FA, Tsai YC, Liang G, Jones PA. Gene. 2002 298:91-9. (Mouse, Human)
[PubMed:12406579]
3
DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR. Nature genetics. 2003 33:61-5. (WB; Human)
[PubMed:12496760]
4
Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells. Qiu YY, Mirkin BL, Dwivedi RS. Cancer detection and prevention. 2002 26:444-53. (ICC, WB; Mouse)
[PubMed:12507229]
5
Histone deacetylase inhibitors decrease DNA methyltransferase-3B messenger RNA stability and down-regulate de novo DNA methyltransferase activity in human endometrial cells. Xiong Y, Dowdy SC, Podratz KC, Jin F, Attewell JR, Eberhardt NL, Jiang SW. Cancer research. 2005 65:2684-9. (WB; Human)
[PubMed:15805266]
6
DNA methylation pathway alterations in an autochthonous murine model of prostate cancer. Morey SR, Smiraglia DJ, James SR, Yu J, Moser MT, Foster BA, Karpf AR. Cancer research. 2006 66:11659-67. (WB; Mouse)
[PubMed:17178860]
7
Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model. Morey Kinney SR, Smiraglia DJ, James SR, Moser MT, Foster BA, Karpf AR. Molecular cancer research : MCR. 2008 6:1365-74. (WB; Mouse)
[PubMed:18667590]
[PMC:PMC2835734]
8
SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53. Kim DH, Jung YJ, Lee JE, Lee AS, Kang KP, Lee S, Park SK, Han MK, Lee SY, Ramkumar KM, Sung MJ, Kim W. American journal of physiology. Renal physiology. 2011 301:F427-35.
[PubMed:21593185]
9
Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes. Kinney SM, Chin HG, Vaisvila R, Bitinaite J, Zheng Y, Estve PO, Feng S, Stroud H, Jacobsen SE, Pradhan S. The Journal of biological chemistry. 2011 286:24685-93. (WB; Mouse)
[PubMed:21610077]
[PMC:PMC3137044]
10
Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL. Kaminskyy VO, Surova OV, Vaculova A, Zhivotovsky B. Carcinogenesis. 2011 32:1450-8.
[PubMed:21771726]
11
DNA methyltransferase inhibitor, zebularine, delays tumor growth and induces apoptosis in a genetically engineered mouse model of breast cancer. Chen M, Shabashvili D, Nawab A, Yang SX, Dyer LM, Brown KD, Hollingshead M, Hunter KW, Kaye FJ, Hochwald SN, Marquez VE, Steeg P, Zajac-Kaye M. Molecular cancer therapeutics. 2012 11:370-82.
[PubMed:22203734]
About DNMT3B
Q9UBC3
NM_006892
NP_008823.1
DNMT3B Antibody, DNA MTase HsaIIIB Antibody, ICF Antibody, ICF1 Antibody, M.HsaIIIB Antibody, DNA methyltransferase HsaIIIB Antibody
PDF Spec Sheet
Technical HelpDNMT3B Antibodies (15)
| Target | Host | Clonality | Format | Epitope | Reactivity | Applications | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DNMT3B | Rabbit | Polyclonal | Human | ELISA, IF, IHC-P, WB |
|
||||||||||||
| DNMT3B | Rabbit | Polyclonal | Human, Mouse | IHC-P, IP, WB |
|
||||||||||||
| DNMT3B | Rabbit | Monoclonal | Human, Mouse, Rat | ICC, WB |
|
||||||||||||
| DNMT3B | Rabbit | Polyclonal | Human | IHC-P, WB |
|
||||||||||||
| DNMT3B | Rabbit | Polyclonal | Human | IF, IHC, WB |
|
||||||||||||
| DNMT3B | Mouse | Monoclonal | Human, Mouse | ChrIP, ICC, IHC-P, IP, WB |
|
||||||||||||
| DNMT3B | Chicken | Polyclonal | Human, Mouse | IHC, WB |
|
||||||||||||
| DNMT3B | Mouse | Monoclonal | Human, Mouse, Rat | DB, Flo, ICC, WB |
|
||||||||||||
| DNMT3B | Rabbit | Monoclonal | Human, Mouse, Rat | ICC, WB |
|
||||||||||||
| DNMT3B | Rabbit | Polyclonal | Bovine, Chicken, Human, Mouse, Pig, Rat, Zebrafish | WB |
|
||||||||||||
| DNMT3B | Rabbit | Polyclonal | Human | ELISA |
|
||||||||||||
| DNMT3B | Mouse | Monoclonal | Human, Mouse | ICC, IF, IP, WB |
|
||||||||||||
| DNMT3B | Mouse | Monoclonal | Mouse | ICC, IP, WB |
|
||||||||||||
| DNMT3B | Rabbit | Polyclonal | Human, Mouse | IP, WB |
|
||||||||||||
| DNMT3B | Mouse | Monoclonal | Human, Mouse, Rat | WB |
|
|
Requested From: United States
Date Requested: 5/21/2013
Date Requested: 5/21/2013