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See All 53 Collagen I Antibodies

Ordering
Wt. Vol. Conc. Price
50 µg - 1 mg/ml $385

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

Collagen I Antibody LS-B342 Images

Immunohistochemistry
Immunohistochemistry of Collagen I antibody.
Immunohistochemistry
Anti-Collagen I antibody IHC staining of human skin.
Western Blot
Anti-Collagen I Antibody - Western Blot.
 

Anti-Collagen I Antibody LS-B342 IHC-plus™

Rabbit anti-Human Polyclonal

100% Guaranteed 100% Guaranteed Publications Publications (4)

Details

Applications
Human Collagen I
Rabbit
Human, Bovine, Mouse, Rat
Polyclonal
Immunoaffinity Chromatography
IHC-P (2.5 µg/ml)
Western blot (1:5000 - 1:50000)
IP
ELISA (1:5000 - 1:50000)
Optimal dilution to be determined by the researcher.

Specificity and Use

Collagen Type I from human and bovine placenta.
Immunohistochemistry: LS-B342 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B342 was determined to be 2.5 ug/ml.

Packaging

0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (4)

1
Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy. Stefanovic B, Schnabl B, Brenner DA. The Journal of biological chemistry. 2002 277:18229-37. [PubMed:11889120]
2
Bone marrow-derived progenitor cells in pulmonary fibrosis. Hashimoto N, Jin H, Liu T, Chensue SW, Phan SH. The Journal of clinical investigation. 2004 113:243-52. [PubMed:14722616] [PMC:PMC310750]
3
Adipogenic transcriptional regulation of hepatic stellate cells. She H, Xiong S, Hazra S, Tsukamoto H. The Journal of biological chemistry. 2005 280:4959-67. [PubMed:15537655]
4
Hepatic steatosis, fibrosis, and cancer in elderly cadavers. Mak KM, Kwong AJ, Chu E, Hoo NM. Anatomical record (Hoboken, N.J. : 2007). 2012 295:40-50. [PubMed:22139908]
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About Collagen I

(More About Collagen I)

Collagen I Antibodies (53)

Target Host Clonality Format Epitope Reactivity Applications
Collagen I Rabbit Polyclonal Bovine, Human, Mouse, Rat ELISA, IHC-P, IP, WB
Collagen I Antibody LS-B342 Rabbit Polyclonal thumb(3)
50 µg - 385.00
Applications: IHC-P, WB, IP... Epitope:  
Reactivity: Bo, Hu, Mu ... Format:  
Collagen I Mouse Monoclonal Bovine, Deer, Human, Rabbit, Rat ELISA, IF, IHC-Fr, IHC-P, IHC-Pfa, WB
Collagen I Antibody LS-B6723 Mouse Monoclonal thumb(1)
50 µl - 385.00
Applications: IHC-Pfa, IHC-P, IHC-Fr... Epitope:  
Reactivity: Bo, De, Hu ... Format:  
Collagen I Rabbit Polyclonal Human ELISA, IF, IHC-P
Collagen I Antibody LS-B3653 Rabbit Polyclonal thumb(3)
125 µl - 385.00
Applications: IHC-P, IF, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IF, IHC-Fr, IHC-P, RIA
Collagen I Antibody LS-B7923 Rabbit Polyclonal thumb(1)
50 µl - 385.00
Applications: IHC-Fr, IHC-P, IF... Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Bovine, Human, Mouse, Rat ELISA, IHC-P, IP, WB
Collagen I Antibody LS-B209 Rabbit Polyclonal thumb(2)
50 µg - 385.00
Applications: IHC-P, WB, IP... Epitope:  
Reactivity: Bo, Hu, Mu ... Format: Biotin  
Collagen I Mouse Monoclonal Human, Sheep ELISA, Flo, IHC-Fr, WB
Collagen I Antibody LS-C79516 Mouse Monoclonal
100 µg - 480.00
Applications: IHC-Fr, WB, Flo... Epitope: C-Terminus  
Reactivity: Hu, Sp Format:  
Collagen I Rabbit Polyclonal Mouse ELISA, IF, IHC-Fr, IHC-P, RIA
Collagen I Antibody LS-C41834 Rabbit Polyclonal
100 µl - 375.00
Applications: IHC-Fr, IHC-P, IF... Epitope:  
Reactivity: Mu Format:  
Collagen I Mouse Monoclonal Human DB, ELISA, IHC-Fr
Collagen I Antibody [NFI/20] LS-C27160 Mouse Monoclonal
100 µg - 295.00
Applications: IHC-Fr, DB, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Rat ELISA, ICC, IHC-Fr
Collagen I Antibody LS-C121633 Rabbit Polyclonal
1000 µl - 475.00
Applications: IHC-Fr, ICC, ELISA Epitope:  
Reactivity: Rt Format:  
Collagen I Rabbit Polyclonal Human ELISA, IHC-Fr, IHC-P
Collagen I Antibody LS-C27161 Rabbit Polyclonal
50 µg - 435.00
Applications: IHC-Fr, IHC-P, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Chicken ELISA, IF, IHC-Fr, IHC-P, RIA
Collagen I Antibody LS-C79498 Rabbit Polyclonal
100 µl - 720.00
Applications: IHC-Fr, IHC-P, IF... Epitope:  
Reactivity: Ck Format:  
Collagen I Rabbit Polyclonal Rat ELISA, IF, IHC-Fr, IHC-P, RIA
Collagen I Antibody LS-C79507 Rabbit Polyclonal
500 µl - 695.00
Applications: IHC-Fr, IHC-P, IF... Epitope:  
Reactivity: Rt Format:  
Collagen I Rabbit Polyclonal Human ELISA, IF, IHC-Fr, IHC-P, RIA
Collagen I Antibody LS-C79514 Rabbit Polyclonal
100 µg - 470.00
Applications: IHC-Fr, IHC-P, IF... Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Chicken ELISA, IHC-Fr, IHC-P, RIA
Collagen I Antibody LS-C79513 Rabbit Polyclonal
100 µg - 475.00
Applications: IHC-Fr, IHC-P, ELISA... Epitope:  
Reactivity: Ck Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr, IHC-P, WB
Collagen I Antibody LS-C123282 Mouse Monoclonal
100 µg - 740.00
Applications: IHC-Fr, IHC-P, WB... Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr, IHC-P, WB
Collagen I Antibody LS-C123278 Mouse Monoclonal
100 µg - 410.00
Applications: IHC-Fr, IHC-P, WB... Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Rat ELISA, IF, IHC-Fr, IHC-P
Collagen I Antibody LS-C123279 Rabbit Polyclonal
500 µl - 465.00
Applications: IHC-Fr, IHC-P, IF... Epitope:  
Reactivity: Rt Format:  
Collagen I Goat Polyclonal Human ELISA, IHC-Fr, IHC-P
Collagen I Antibody LS-C123276 Goat Polyclonal
200 µg - 530.00
Applications: IHC-Fr, IHC-P, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr, IHC-P
Collagen I Antibody LS-C123277 Mouse Monoclonal
100 µg - 530.00
Applications: IHC-Fr, IHC-P, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IHC-Fr, IHC-P
Collagen I Antibody LS-C121628 Rabbit Polyclonal
50 µg - 560.00
Applications: IHC-Fr, IHC-P, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human IHC-Fr, IHC-P
Collagen I Antibody LS-C123280 Mouse Monoclonal
100 µg - 480.00
Applications: IHC-Fr, IHC-P Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Rat ELISA, IF, IHC-Fr
Collagen I Antibody LS-C41836 Rabbit Polyclonal
500 µl - 345.00
Applications: IHC-Fr, IF, ELISA Epitope:  
Reactivity: Rt Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr
Collagen I Antibody [2F2/51] LS-C27159 Mouse Monoclonal
100 µg - 410.00
Applications: IHC-Fr, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Goat Polyclonal Human ELISA, IHC-Fr
Collagen I Antibody LS-C41769 Goat Polyclonal
200 µg - 410.00
Applications: IHC-Fr, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IF, IHC-Fr, IP, WB
Collagen I Antibody LS-C79488 Rabbit Polyclonal
50 µg - 480.00
Applications: IHC-Fr, IF, WB... Epitope:  
Reactivity: Hu Format: Biotin  
Collagen I Rabbit Polyclonal Human ELISA, IHC-Fr, IP, WB
Collagen I Antibody LS-C79489 Rabbit Polyclonal
100 µg - 310.00
Applications: IHC-Fr, WB, IP... Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IHC-Fr, IP, WB
Collagen I Antibody LS-C79490 Rabbit Polyclonal
50 µg - 360.00
Applications: IHC-Fr, WB, IP... Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IHC, IP, WB
Collagen I Antibody LS-C79491 Rabbit Polyclonal
100 µg - 470.00
Applications: IHC, WB, IP... Epitope:  
Reactivity: Hu Format: Biotin  
Collagen I Rabbit Polyclonal Bovine ELISA, IF, IHC-Fr, RIA
Collagen I Antibody LS-C79494 Rabbit Polyclonal
500 µl - 655.00
Applications: IHC-Fr, IF, ELISA... Epitope:  
Reactivity: Bo Format:  
Collagen I Goat Polyclonal Human ELISA, ICC, IHC-Fr, WB
Collagen I Antibody LS-C79501 Goat Polyclonal
200 µg - 560.00
Applications: IHC-Fr, ICC, WB... Epitope:  
Reactivity: Hu Format: Biotin  
Collagen I Rabbit Polyclonal Human, Mouse ELISA, IF, IHC-P, RIA
Collagen I Antibody LS-C79504 Rabbit Polyclonal
100 µl - 590.00
Applications: IHC-P, IF, ELISA... Epitope:  
Reactivity: Hu, Mu Format:  
Collagen I Rabbit Polyclonal Mouse ELISA, IF, IHC-P, RIA
Collagen I Antibody LS-C79497 Rabbit Polyclonal
500 µl - 700.00
Applications: IHC-P, IF, ELISA... Epitope:  
Reactivity: Mu Format:  
Collagen I Goat Polyclonal Human ELISA, ICC, IHC-Fr, WB
Collagen I Antibody LS-C79509 Goat Polyclonal
200 µg - 680.00
Applications: IHC-Fr, ICC, WB... Epitope:  
Reactivity: Hu Format: Biotin  
Collagen I Rabbit Polyclonal Mouse ELISA, IHC-Fr, RIA, WB
Collagen I Antibody LS-C79510 Rabbit Polyclonal
100 µg - 520.00
Applications: IHC-Fr, WB, ELISA... Epitope:  
Reactivity: Mu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IF, IHC-Fr, WB
Collagen I Antibody LS-C121630 Rabbit Polyclonal
1000 µl - 395.00
Applications: IHC-Fr, IF, WB... Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, ICC, IHC-Fr, WB
Collagen I Antibody LS-C121631 Rabbit Polyclonal
1000 µl - 475.00
Applications: IHC-Fr, ICC, WB... Epitope:  
Reactivity: Hu Format:  
Collagen I Goat Polyclonal Human DB, ELISA, ICC, IHC-Fr
Collagen I Antibody LS-C121632 Goat Polyclonal
1000 µl - 475.00
Applications: IHC-Fr, ICC, DB... Epitope:  
Reactivity: Hu Format:  
Collagen I Goat Polyclonal Human ELISA, ICC, IHC-Fr
Collagen I Antibody LS-C79511 Goat Polyclonal
200 µg - 535.00
Applications: IHC-Fr, ICC, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Rat ELISA, IHC-Fr, RIA
Collagen I Antibody LS-C79512 Rabbit Polyclonal
100 µg - 470.00
Applications: IHC-Fr, ELISA, RIA Epitope:  
Reactivity: Rt Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr, RIA
Collagen I Antibody LS-C79517 Mouse Monoclonal
100 µg - 410.00
Applications: IHC-Fr, ELISA, RIA Epitope:  
Reactivity: Hu Format:  
Collagen I Goat Polyclonal Human ELISA, ICC, IHC-Fr
Collagen I Antibody LS-C79518 Goat Polyclonal
200 µg - 595.00
Applications: IHC-Fr, ICC, ELISA Epitope:  
Reactivity: Hu Format: Biotin  
Collagen I Rabbit Polyclonal Salmon ELISA, IF, IHC-Fr
Collagen I Antibody LS-C79499 Rabbit Polyclonal
500 µl - 730.00
Applications: IHC-Fr, IF, ELISA Epitope:  
Reactivity: Fi Format:  
Collagen I Goat Polyclonal Human ELISA, ICC, IHC-Fr
Collagen I Antibody LS-C79500 Goat Polyclonal
200 µg - 470.00
Applications: IHC-Fr, ICC, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Human ELISA, IHC-Fr, WB
Collagen I Antibody LS-C79492 Rabbit Polyclonal
100 µg - 475.00
Applications: IHC-Fr, WB, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Goat Polyclonal Human ELISA, ICC, IHC-Fr
Collagen I Antibody LS-C79493 Goat Polyclonal
200 µg - 545.00
Applications: IHC-Fr, ICC, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr
Collagen I Antibody LS-C79508 Mouse Monoclonal
100 µg - 560.00
Applications: IHC-Fr, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human ELISA, IHC-Fr
Collagen I Antibody LS-C123281 Mouse Monoclonal
100 µg - 695.00
Applications: IHC-Fr, ELISA Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human IHC-Fr
Collagen I Antibody LS-C79495 Mouse Monoclonal
100 µg - 445.00
Applications: IHC-Fr Epitope:  
Reactivity: Hu Format:  
Collagen I Mouse Monoclonal Human IHC-Fr
Collagen I Antibody LS-C79496 Mouse Monoclonal
100 µg - 445.00
Applications: IHC-Fr Epitope:  
Reactivity: Hu Format:  
Collagen I Rabbit Polyclonal Chicken ELISA, IF
Collagen I Antibody LS-C41833 Rabbit Polyclonal
500 µl - 385.00
Applications: IF, ELISA Epitope:  
Reactivity: Ck Format:  
Collagen I Goat Polyclonal Human ELISA
Collagen I Antibody LS-C41770 Goat Polyclonal
200 µg - 535.00
Applications: ELISA Epitope:  
Reactivity: Hu Format: Biotin  
Collagen I Mouse Monoclonal Human, Rabbit
Collagen I Antibody [I-8H5] LS-C84855 Mouse Monoclonal
100 µg - 595.00
Applications: Epitope:  
Reactivity: Hu, Rb Format:  
Collagen I Rabbit Polyclonal Chicken ELISA, IF
Collagen I Antibody LS-C128243 Rabbit Polyclonal
500 µl - 495.00
Applications: IF, ELISA Epitope:  
Reactivity: Ck Format:  

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: No antigen retrieval was performed. Primary antibody: Collagen I 1:100 4 hours at room temperature Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen I antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B342 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-Collagen I Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 ug/10 ml. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences). Other detection systems will yield similar results. See Hazra et al. (2004) for additional details.
  
Requested From: United States
Date Requested: 6/20/2013
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