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Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $225

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

B7-H1 / PD-L1 / CD274 Antibody LS-C58199 Images

Flow Cytometry
Mouse lymphocytes stained with Rat anti Mouse CD274.

Anti-B7-H1 / PD-L1 / CD274 Antibody LS-C58199

Rat anti-Mouse Monoclonal, Clone MIH6, Unconjugated

100% Guaranteed 100% Guaranteed

Details

Applications

Mouse B7-H1 / PD-L1 / CD274
Rat
Mouse
IgG2a Monoclonal [MIH6]
Affinity Chromatography
Unconjugated (Unconjugated)
IHC-Fr
IP
Flo (1/1 - 1/10)
Optimal dilution to be determined by the researcher.
Immunohistochemistry - Paraffin (IHC-P)

Specificity and Use

B7-H1 / PD-L1 / CD274 antibody was raised against mouse CD274 - transfected L5178Y cells.
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About B7-H1 / PD-L1 / CD274

Q9NZQ7 NM_014143 NP_054862.1

CD274 Antibody, B7-H Antibody, B7 homolog 1 Antibody, B7-H1 Antibody, B7H1 Antibody, CD274 molecule Antibody, CD274 antigen Antibody, PDCD1 ligand 1 Antibody, PDL1 Antibody, PD-L1 Antibody, PDCD1L1 Antibody, PDCD1LG1 Antibody, Programmed death ligand 1 Antibody

CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein, is a member of the B7 family of co-stimulatory molecules.


B7-H1 / PD-L1 / CD274 Antibodies (25)

Target Host Clonality Format Epitope Reactivity Applications
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human IHC-P, WB
B7-H1 / PD-L1 / CD274 Antibody LS-B480 Rabbit Polyclonal thumb(1)
50 µg - 385.00
Applications: IHC-P, WB Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Goat Polyclonal Human ELISA, IHC-P, WB
B7-H1 / PD-L1 / CD274 Antibody LS-B2999 Goat Polyclonal thumb(3)
50 µg - 385.00
Applications: IHC-P, WB, ELISA Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [130021] LS-C128031 Mouse Monoclonal
100 µg - 455.00
Applications: Flo, ELISA Epitope: aa19-338  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human WB
B7-H1 / PD-L1 / CD274 Antibody LS-C146478 Rabbit Polyclonal thumb(1)
50 µg - 380.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH6] LS-C58198 Rat Monoclonal thumb(1)
100 µg - 325.00
Applications: Flo Epitope:  
Reactivity: Mu Format: FITC  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [ANC6H1] LS-C134636 Mouse Monoclonal
100 µg - 340.00
Applications: Flo, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [ANC6H1] LS-C134637 Mouse Monoclonal
100 µg - 430.00
Applications: Flo, ELISA Epitope:  
Reactivity: Hu Format: Biotin  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [ANC6H1] LS-C134638 Mouse Monoclonal
100 µg - 340.00
Applications: Flo, ELISA Epitope:  
Reactivity: Hu Format: Azide-free  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody LS-C128033 Mouse Monoclonal
100 µg - 425.00
Applications: Flo Epitope:  
Reactivity: Hu Format: FITC  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo, IHC-Fr, IP
B7-H1 / PD-L1 / CD274 Antibody [MIH6] LS-C58199 Rat Monoclonal thumb(1)
100 µg - 225.00
Applications: IHC-Fr, Flo, IP Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo, IHC-P
B7-H1 / PD-L1 / CD274 Antibody [27A2] LS-C179481 Mouse Monoclonal thumb(1)
100 µg - 340.00
Applications: IHC-P, Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Goat Polyclonal Mouse ELISA, Flo, IHC, WB
B7-H1 / PD-L1 / CD274 Antibody LS-C150153 Goat Polyclonal
100 µg - 395.00
Applications: IHC, WB, Flo... Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo, IHC-Fr, IP
B7-H1 / PD-L1 / CD274 Antibody LS-C123195 Rat Monoclonal
250 µg - 410.00
Applications: IHC-Fr, Flo, IP Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo, IHC-Fr, IP
B7-H1 / PD-L1 / CD274 Antibody LS-C123196 Rat Monoclonal
100 µg - 335.00
Applications: IHC-Fr, Flo, IP Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody [1-111A] LS-C106660 Rat Monoclonal thumb(2)
50 µg - 240.00
100 µg - 310.00
500 µg - 465.00
Applications: Flo Epitope:  
Reactivity: Mu Format: Biotin  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody [1-111A] LS-C107317 Rat Monoclonal thumb(1)
50 µg - 230.00
100 µg - 290.00
500 µg - 450.00
Applications: Flo Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH2] LS-C58200 Mouse Monoclonal thumb(1)
100 µg - 255.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [29E.2A3] LS-C40827 Mouse Monoclonal thumb(1)
100 µg - 225.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human WB
B7-H1 / PD-L1 / CD274 Antibody LS-C168531 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: aa260-290  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human WB
B7-H1 / PD-L1 / CD274 Antibody LS-C169740 Rabbit Polyclonal thumb(1)
50 µg - 365.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH3] LS-C179365 Mouse Monoclonal thumb(1)
1000 µl - 355.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Biotin  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [27A2] LS-C179482 Mouse Monoclonal
50 tst - 340.00
Applications: Flo Epitope:  
Reactivity: Hu Format: PE  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH3] LS-C179364 Mouse Monoclonal
100 µg - 340.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody LS-C128034 Rat Monoclonal
100 µg - 335.00
Applications: Flo Epitope:  
Reactivity: Mu Format: FITC  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody LS-C125334 Mouse Monoclonal
100 tst - 435.00
Applications: Flo Epitope:  
Reactivity: Hu Format: PE  

Flow Cytometry

Flow Cytometry
Mouse lymphocytes stained with Rat anti Mouse CD274.
 

Requested From: United States
Date Requested: 5/23/2013
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